Publication detail

Evaluations of methods for DNA isolation from plant samples

TROJÁNEK, Z., HÚSKA, D., ŠPANOVÁ, A., KOVAŘÍK, A., RITTICH, B.

Original Title

Evaluations of methods for DNA isolation from plant samples

Type

abstract

Language

English

Original Abstract

The aim of this study was to evaluate CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration and purity, relative intactness, amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. Here we provide an optimized protocol for isolation of DNA from different plant species by combination of direct homogenization and magnetic microspheres coated by carboxyl groups.

Keywords

magnetic particles, plants, foods, polymerase chain reaction, identification

Authors

TROJÁNEK, Z., HÚSKA, D., ŠPANOVÁ, A., KOVAŘÍK, A., RITTICH, B.

Released

28. 5. 2013

Publisher

Institute of Immunolology and Experimental Therapy PASci

Location

Polsko

ISBN

978-83-928488-3-7

Book

Microbiology and Immunology of Mucosa Probiotics Conference 2013

Edition number

1

Pages from

45

Pages to

45

Pages count

1

BibTex

@misc{BUT100203,
  author="Bohuslav {Rittich}",
  title="Evaluations of methods for DNA isolation from plant samples",
  booktitle="Microbiology and Immunology of Mucosa Probiotics Conference 2013",
  year="2013",
  edition="1",
  pages="45--45",
  publisher="Institute of Immunolology and Experimental Therapy PASci",
  address="Polsko",
  isbn="978-83-928488-3-7",
  note="abstract"
}