Detail publikace

Directionality of developing skeletal muscles is set by mechanical forces

Sunadome, K. Erickson, A.G. Kah, D. Fabry, B. Adori, C. Kameneva, P. Faure, L. Kanatani, S. Kaucka, M. Ellström, I.D. Tesarova, M. Zikmund, T. Kaiser, J. Edwards, S. Maki, K. Adachi, T. Yamamoto, T. Fried K. Adameyko, I.

Originální název

Directionality of developing skeletal muscles is set by mechanical forces

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The mechanisms that drive myocyte orientation and fusion to control muscle directionality are not well understood. Here authors show that the developing skeleton produces mechanical tension that instructs the directional outgrowth of skeletal muscles. Formation of oriented myofibrils is a key event in musculoskeletal development. However, the mechanisms that drive myocyte orientation and fusion to control muscle directionality in adults remain enigmatic. Here, we demonstrate that the developing skeleton instructs the directional outgrowth of skeletal muscle and other soft tissues during limb and facial morphogenesis in zebrafish and mouse. Time-lapse live imaging reveals that during early craniofacial development, myoblasts condense into round clusters corresponding to future muscle groups. These clusters undergo oriented stretch and alignment during embryonic growth. Genetic perturbation of cartilage patterning or size disrupts the directionality and number of myofibrils in vivo. Laser ablation of musculoskeletal attachment points reveals tension imposed by cartilage expansion on the forming myofibers. Application of continuous tension using artificial attachment points, or stretchable membrane substrates, is sufficient to drive polarization of myocyte populations in vitro. Overall, this work outlines a biomechanical guidance mechanism that is potentially useful for engineering functional skeletal muscle.

Klíčová slova

muscles, myocyte, morphogenesis, cartilage development, cell polarity, skeletal muscle

Autoři

Sunadome, K.; Erickson, A.G.; Kah, D.; Fabry, B.; Adori, C.; Kameneva, P.; Faure, L.; Kanatani, S.; Kaucka, M.; Ellström, I.D.; Tesarova, M.; Zikmund, T.; Kaiser, J.; Edwards, S.; Maki, K.; Adachi, T.; Yamamoto, T.; Fried K.; Adameyko, I.

Vydáno

27. 5. 2023

Nakladatel

NATURE PORTFOLIO

Místo

BERLIN

ISSN

2041-1723

Periodikum

NATURE COMMUNICATIONS

Ročník

14

Číslo

3060

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

24

Strany počet

24

URL

https://www.nature.com/articles/s41467-023-38647-7

BibTex

@article{BUT185233,
  author="Sunadome, K. and Erickson, A.G. and Kah, D. and Fabry, B. and Adori, C. and Kameneva, P. and Faure, L. and Kanatani, S. and Kaucka, M. and Ellström, I.D. and Tesarova, M. and Zikmund, T. and Kaiser, J. and Edwards, S. and Maki, K. and Adachi, T. and Yamamoto, T. and Fried K. and Adameyko, I.",
  title="Directionality of developing skeletal muscles is set by mechanical forces",
  journal="NATURE COMMUNICATIONS",
  year="2023",
  volume="14",
  number="3060",
  pages="1--24",
  doi="10.1038/s41467-023-38647-7",
  issn="2041-1723",
  url="https://www.nature.com/articles/s41467-023-38647-7"
}