Detail publikace

Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging

STIBOREK, M. JINDŘICHOVÁ, L. MELIORISOVÁ, S. BEDNAŘÍK, A. PRYSIAZHNYI, V. KROUPA, J. HOUŠKA, P. ADAMOVÁ, B. NAVRÁTILOVÁ, J. KANICKÝ, V. PREISLER, J.

Originální název

Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.

Klíčová slova

Desorption; Fluorescence microscopy; Gold nanoparticles; Inductively coupled plasma mass spectrometry; Infrared lasers; Laser ablation; Mapping; Mass spectrometers; Medical applications; Tissue

Autoři

STIBOREK, M.; JINDŘICHOVÁ, L.; MELIORISOVÁ, S.; BEDNAŘÍK, A.; PRYSIAZHNYI, V.; KROUPA, J.; HOUŠKA, P.; ADAMOVÁ, B.; NAVRÁTILOVÁ, J.; KANICKÝ, V.; PREISLER, J.

Vydáno

27. 12. 2022

Nakladatel

AMER CHEMICAL SOC

Místo

WASHINGTON

ISSN

0003-2700

Periodikum

ANALYTICAL CHEMISTRY

Ročník

94

Číslo

51

Stát

Spojené státy americké

Strany od

18114

Strany do

18120

Strany počet

7

URL

https://pubs-acs-org.ezproxy.lib.vutbr.cz/doi/full/10.1021/acs.analchem.2c05216

BibTex

@article{BUT182555,
  author="Marek {Stiborek} and Lenka {Jindřichová} and Stanislava {Meliorisová} and Antonín {Bednařík} and Vadym {Prysiazhnyi} and Jiří {Kroupa} and Pavel {Houška} and Barbora {Adamová} and Jarmila {Navrátilová} and Viktor {Kanický} and Jan {Preisler}",
  title="Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging",
  journal="ANALYTICAL CHEMISTRY",
  year="2022",
  volume="94",
  number="51",
  pages="18114--18120",
  doi="10.1021/acs.analchem.2c05216",
  issn="0003-2700",
  url="https://pubs-acs-org.ezproxy.lib.vutbr.cz/doi/full/10.1021/acs.analchem.2c05216"
}