Detail publikace

Digital PCR system development accelerator-A methodology to emulate dPCR results

ZHANG, H. YAN, Z. WANG, X. GAŇOVÁ, M. KORABEČNÁ, M. ZAHRADNÍK, P. CHANG, H. NEUŽIL, P.

Originální název

Digital PCR system development accelerator-A methodology to emulate dPCR results

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The development of a digital polymerase chain reaction (dPCR) system typically begins with an idea for the system configuration and chip layout, followed by design, fabrication, and hardware testing. The image processing software can be developed and verified based on the test results. In this paper, we proposed a dPCR emulation methodology to train the developed image processing software before building the dPCR system hardware. We developed a script in a MATLAB environment to generate artificial dPCR images and emulate the dPCR results. First, we defined the number of parameters corresponding to the emulated results, such as the number of partitions with targets, background fluorescence distribution and intensity, image defects, image rotation angle, shift, non-uniform light distribution, and temperature sensitivity. We then implemented the defined parameters and generated an artificial dPCR chip image based on layout design or pattern recognition algorithm. Finally, we obtained a dataset from the artificial image for subsequent result analysis. The generated images could then be used to train the image processing algorithms based on the requirements. We verified the proposed method using various designs of dPCR chips from recently published papers, demonstrating the method's versatility. The proposed method also demonstrated the capability for separating the software and hardware development. Thus, our method allowed the image processing and hardware to be concurrently designed and tested simplifying and speeding up the dPCR system development.

Klíčová slova

Digital polymerase chain reaction; Image emulation; Gauss distribution; Poisson distribution; MATLAB

Autoři

ZHANG, H.; YAN, Z.; WANG, X.; GAŇOVÁ, M.; KORABEČNÁ, M.; ZAHRADNÍK, P.; CHANG, H.; NEUŽIL, P.

Vydáno

1. 5. 2022

Nakladatel

ELSEVIER SCIENCE SA

Místo

LAUSANNE

ISSN

0925-4005

Periodikum

Sensors and Actuators B: Chemical

Ročník

358

Číslo

131527

Stát

Švýcarská konfederace

Strany počet

10

URL

https://www.sciencedirect.com/science/article/pii/S0925400522001691?via%3Dihub

BibTex

@article{BUT182319,
  author="Haoqing {Zhang} and Zhiqiang {Yan} and Xinlu {Wang} and Martina {Gaňová} and Marie {Korabečná} and Pavel {Zahradník} and Honglong {Chang} and Pavel {Neužil}",
  title="Digital PCR system development accelerator-A methodology to emulate dPCR results",
  journal="Sensors and Actuators B: Chemical",
  year="2022",
  volume="358",
  number="131527",
  pages="10",
  doi="10.1016/j.snb.2022.131527",
  issn="0925-4005",
  url="https://www.sciencedirect.com/science/article/pii/S0925400522001691?via%3Dihub"
}