Detail publikace

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

VODIČKA, J. WIKARSKÁ, M. TRUDIČOVÁ, M. JUGLOVÁ, Z. POSPÍŠILOVÁ, A. KALINA, M. SLANINOVÁ, E. OBRUČA, S. SEDLÁČEK, P.

Originální název

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate-P(3HB-co-36 mol.% 4HB) and P(3HB-co-66 mol.% 4HB), by cultivating the thermophilic bacterial strain Aneurinibacillus sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-co-4HB) copolymers produced by Aneurinibacillus sp. H1 as being very auspicious polymers for intestinal in vivo treatments.

Klíčová slova

polyhydroxyalkanoates (PHA); Aneurinibacillus sp. H1; PHA copolymers; biodegradation; simulated body fluids

Autoři

VODIČKA, J.; WIKARSKÁ, M.; TRUDIČOVÁ, M.; JUGLOVÁ, Z.; POSPÍŠILOVÁ, A.; KALINA, M.; SLANINOVÁ, E.; OBRUČA, S.; SEDLÁČEK, P.

Vydáno

13. 5. 2022

Nakladatel

MDPI

Místo

BASEL

ISSN

2073-4360

Periodikum

Polymers

Ročník

14

Číslo

10

Stát

Švýcarská konfederace

Strany od

1

Strany do

14

Strany počet

14

URL

https://www.mdpi.com/2073-4360/14/10/1990

Plný text v Digitální knihovně

http://hdl.handle.net/11012/204434

BibTex

@article{BUT177660,
  author="Juraj {Vodička} and Monika {Wikarská} and Monika {Trudičová} and Zuzana {Juglová} and Aneta {Pospíšilová} and Michal {Kalina} and Eva {Slaninová} and Stanislav {Obruča} and Petr {Sedláček}",
  title="Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids",
  journal="Polymers",
  year="2022",
  volume="14",
  number="10",
  pages="1--14",
  doi="10.3390/polym14101990",
  issn="2073-4360",
  url="https://www.mdpi.com/2073-4360/14/10/1990"
}