Detail publikace

CYTOTOXIC AND GENOTOXIC EFFECTS OF PHA/LIPOSOME PARTICLES WITH ENCAPSULATED EXTRACTS FROM COFFEA ARABICA

Originální název

CYTOTOXIC AND GENOTOXIC EFFECTS OF PHA/LIPOSOME PARTICLES WITH ENCAPSULATED EXTRACTS FROM COFFEA ARABICA

Anglický název

CYTOTOXIC AND GENOTOXIC EFFECTS OF PHA/LIPOSOME PARTICLES WITH ENCAPSULATED EXTRACTS FROM COFFEA ARABICA

Jazyk

en

Originální abstrakt

Plants are sources of broad spectrum of compounds potentially useable as antioxidants and UV protectants in cosmetics. This work aims at extraction and preservation of chosen plant extracts into combined PHA/phospholipid nanoparticles to prolong their activity and stability. Extracts were characterized in terms of polyphenols, flavonoids, antioxidant activity, UV protective properties and packed into liposomes. Encapsulation efficiency of PHA/liposomes was characterized by spectrophotometry. Particle size and size distribution were observed by dynamic light scattering and colloid stability by determining zeta-potential. Safety of prepared particles with plant extracts were tested by MTT and LDH assay on human keratinocytes. Influence of functionalized particles on cell viability was evaluated. Genotoxicity was tested using SOS ChromotestTM. All the prepared samples showed moderate or high encapsulation efficiency (40–97 %). Extracted active compounds had high antioxidant activity as well as UV protective effect. The most of prepared particles did not posses cytotoxic effect on human keratinocytes. No genotoxicity was found in tested particles. Encapsulation of active compounds from plants into combined PHA/lipomes can be an interesting way how to prolog their activity. Besides UV protecting effect for natural cosmetics they can find use as preserves in cosmetics as well as in food industry.

Anglický abstrakt

Plants are sources of broad spectrum of compounds potentially useable as antioxidants and UV protectants in cosmetics. This work aims at extraction and preservation of chosen plant extracts into combined PHA/phospholipid nanoparticles to prolong their activity and stability. Extracts were characterized in terms of polyphenols, flavonoids, antioxidant activity, UV protective properties and packed into liposomes. Encapsulation efficiency of PHA/liposomes was characterized by spectrophotometry. Particle size and size distribution were observed by dynamic light scattering and colloid stability by determining zeta-potential. Safety of prepared particles with plant extracts were tested by MTT and LDH assay on human keratinocytes. Influence of functionalized particles on cell viability was evaluated. Genotoxicity was tested using SOS ChromotestTM. All the prepared samples showed moderate or high encapsulation efficiency (40–97 %). Extracted active compounds had high antioxidant activity as well as UV protective effect. The most of prepared particles did not posses cytotoxic effect on human keratinocytes. No genotoxicity was found in tested particles. Encapsulation of active compounds from plants into combined PHA/lipomes can be an interesting way how to prolog their activity. Besides UV protecting effect for natural cosmetics they can find use as preserves in cosmetics as well as in food industry.

BibTex


@misc{BUT155293,
  author="Renata {Pavelková} and Petra {Matoušková} and Jitka {Bokrová} and Monika {Plachá} and Matej {Tarageľ} and Ivana {Márová}",
  title="CYTOTOXIC AND GENOTOXIC EFFECTS OF PHA/LIPOSOME PARTICLES WITH ENCAPSULATED EXTRACTS FROM COFFEA ARABICA",
  annote="Plants are sources of broad spectrum of compounds potentially useable as antioxidants and UV protectants in cosmetics.
This work aims at extraction and preservation of chosen plant extracts into combined PHA/phospholipid nanoparticles to prolong their activity and stability. Extracts were characterized in terms of polyphenols, flavonoids, antioxidant activity, UV protective properties and packed into liposomes.
Encapsulation efficiency of PHA/liposomes was characterized by spectrophotometry. Particle size and size distribution were observed by dynamic light scattering and colloid stability by determining zeta-potential. Safety of prepared particles with plant extracts were tested by MTT and LDH assay on human keratinocytes. Influence of functionalized particles on cell viability was evaluated. Genotoxicity was tested using SOS ChromotestTM.
All the prepared samples showed moderate or high encapsulation efficiency (40–97 %). Extracted active compounds had high antioxidant activity as well as UV protective effect. The most of prepared particles did not posses cytotoxic effect on human keratinocytes. No genotoxicity was found in tested particles.
Encapsulation of active compounds from plants into combined PHA/lipomes can be an interesting way how to prolog their activity. Besides UV protecting effect for natural cosmetics they can find use as preserves in cosmetics as well as in food industry.",
  address="AMOS repro Ltd., 1. maje 3236/103, 703 00 Moravska Ostrava, Czech Republic, EU",
  booktitle="NANOCON 2018 - Abstracts",
  chapter="155293",
  edition="1 st edition",
  howpublished="print",
  institution="AMOS repro Ltd., 1. maje 3236/103, 703 00 Moravska Ostrava, Czech Republic, EU",
  year="2018",
  month="october",
  pages="117",
  publisher="AMOS repro Ltd., 1. maje 3236/103, 703 00 Moravska Ostrava, Czech Republic, EU",
  type="abstract"
}