Prostate tumor attenuation in the nu/nu murine model due to antisarcosine antibodies in folate targeted liposomes

článek v časopise ve Web of Science, Jimp

angličtina

Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously added folic acid supported the role of folate targeting in liposome internalization. We examined the effects of repeated administration on mice PC-3 xenografts. Sar@LIP treatment significantly increased tumor volume and weight compared to controls treated with empty liposomes. Moreover, antisarAbs@LIP administration exhibited a mild antitumor effect. We also identified differences in gene expression patterns post-treatment. Furthermore, Sar@LIP treatment resulted in decreased amounts of tumor zinc ions and total metallothioneins. Examination of the spatial distribution across the tumor sections revealed a sarcosine-related decline of the MT1X isoform within the marginal regions but an elevation after antisarAbs@LIP administration. Our exploratory results demonstrate the importance of sarcosine as an oncometabolite in PCa. Moreover, we have shown that sarcosine can be a potential target for anticancer strategies in management of PCa.

Cancer progression; Cell-line; In-vitro; Metallothionein; Expression; Therapy; Protein; Urine; Nanoparticles; Trafficking

HEGER, Z.; POLANSKÁ, H.; MERLOS RODRIGO, M.; GURÁŇ, R.; KULICH, P.; KOPEL, P.; MASAŘÍK, M.; ECKSCHLAGER, T.; STIBOROVÁ, M.; KIZEK, R.; ADAM, V.

20. 9. 2016

Nature Publishing Group

2045-2322

Scientific Reports

6

1

Spojené království Velké Británie a Severního Irska

33379-1

33379-11

11

http://www.nature.com/articles/srep33379

http://hdl.handle.net/11012/63729