Detail publikace

The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses

CHUDOBOVÁ, D. DOSTÁLOVÁ, S. RUTTKAY-NEDECKÝ, B. GURÁŇ, R. MERLOS RODRIGO, M. TMEJOVÁ, K. KŘÍŽKOVÁ, S. ZÍTKA, O. ADAM, V. KIZEK, R.

Originální název

The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

tIn this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacte-ria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions ofheavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochem-ical changes of resistant strains in comparison with the non-resistant control strain of S. aureus wereobserved by microbiological (measuring - growth curves and inhibition zones) and spectrophotometricmethods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phos-phatase, -glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysisof the samples (changes in S. aureus protein composition) and for the identification of the strains databaseMALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition,were observed in resistant strains compared to non-resistant control strain. Our results describe the pos-sible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoringof changes in genetic information caused by the forming of resistance to heavy metals.

Klíčová slova

S. aureus, Resistance, Heavy metals, Mass spectrometry

Autoři

CHUDOBOVÁ, D.; DOSTÁLOVÁ, S.; RUTTKAY-NEDECKÝ, B.; GURÁŇ, R.; MERLOS RODRIGO, M.; TMEJOVÁ, K.; KŘÍŽKOVÁ, S.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

Rok RIV

2015

Vydáno

1. 1. 2015

ISSN

0944-5013

Periodikum

MICROBIOLOGICAL RESEARCH

Ročník

170

Číslo

1

Stát

Spolková republika Německo

Strany od

147

Strany do

156

Strany počet

10

BibTex

@article{BUT113354,
  author="Dagmar {Hegerová} and Simona {Dostálová} and Branislav {Ruttkay-Nedecký} and Roman {Guráň} and Miguel Ángel {Merlos Rodrigo} and Kateřina {Tmejová} and Soňa {Křížková} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses",
  journal="MICROBIOLOGICAL RESEARCH",
  year="2015",
  volume="170",
  number="1",
  pages="147--156",
  doi="10.1016/j.micres.2014.08.003",
  issn="0944-5013"
}