Detail publikace

Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application

MANAKHOV, A. SKLÁDAL, P. NEČAS, D. ČECHAL, J. POLČÁK, J. ELIÁŠ, M. ZAJÍČKOVÁ, L.

Originální název

Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using the self-assembled thiol monolayer based on cystamine.

Klíčová slova

amine plasma; biosensors; polymers; quartz crystal microbalance; X-ray photoelectron spectroscopy

Autoři

MANAKHOV, A.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; POLČÁK, J.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.

Rok RIV

2014

Vydáno

12. 12. 2014

ISSN

1862-6300

Periodikum

physica status solidi (a)

Ročník

211

Číslo

12

Stát

Spolková republika Německo

Strany od

2801

Strany do

2808

Strany počet

8

BibTex

@article{BUT111156,
  author="Anton {Manakhov} and Petr {Skládal} and David {Nečas} and Jan {Čechal} and Josef {Polčák} and Marek {Eliáš} and Lenka {Zajíčková}",
  title="Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application",
  journal="physica status solidi (a)",
  year="2014",
  volume="211",
  number="12",
  pages="2801--2808",
  doi="10.1002/pssa.201431399",
  issn="1862-6300"
}