Detail publikace

Porovnání metod pro vyhodnocení depozice částic v modelu lidských plic

BĚLKA, M. LIPPAY, J. LÍZAL, F. JEDELSKÝ, J. JÍCHA, M.

Originální název

Comparison of methods for evaluation of aerosol deposition in the model of human lungs

Český název

Porovnání metod pro vyhodnocení depozice částic v modelu lidských plic

Anglický název

Comparison of methods for evaluation of aerosol deposition in the model of human lungs

Typ

článek ve sborníku

Jazyk

en

Originální abstrakt

It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.

Český abstrakt

Podávání léků inhalací místo injekční cestou je velice pohodlné. Avšak transport aerosolů a jeho cílená dodávka v lidských dýchacích cestách je velice komplikovaný problém. Proto bylo provedeno několik depozičních experimentů a odzkoušeno několik metod pro vyhodnocení depozice v modelu lidských plic. Model obsahoval dýchací cesty od ústní dutiny až po 7. generaci větvení. Částice byly dispergovány pomocí TSI SSPD 3433 a aplikovány do modelu. Po ukončení depozice byl model rozebrán a depozice v jednotlivých částech byla analyzována. Pro vyhodnocení byly použity 2 metody, fluorescenční spektroskopie a optická mikroskopie. První metoda využívá měření intenzity fluorescence částic, jejíž velikost určuje míru depozice. Druhá metoda využívá optické mikroskopie ve spojení s fázovým kontrastem. Výsledky obou metod byly porovnány a metody byly vyhodnoceny jako použitelné.

Anglický abstrakt

It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.

Klíčová slova

Depozice aerosolu, optická mikroskopie, fluorescenční spektroskopie, dýchací cesty

Rok RIV

2013

Vydáno

25.03.2014

Nakladatel

E D P SCIENCES

Místo

FRANCE

ISBN

978-80-260-5375-0

Kniha

EPJ Web of Conferences

Strany od

82

Strany do

85

Strany počet

4

BibTex


@inproceedings{BUT104093,
  author="Miloslav {Bělka} and Josef {Lippay} and František {Lízal} and Jan {Jedelský} and Miroslav {Jícha}",
  title="Comparison of methods for evaluation of aerosol deposition in the model of human lungs",
  annote="It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.",
  address="E D P SCIENCES",
  booktitle="EPJ Web of Conferences",
  chapter="104093",
  doi="10.1051/epjconf/20146702006",
  howpublished="online",
  institution="E D P SCIENCES",
  number="2014",
  year="2014",
  month="march",
  pages="82--85",
  publisher="E D P SCIENCES",
  type="conference paper"
}