Detail publikace

Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element

POHANKA, M. FUSEK, J. ADAM, V. KIZEK, R.

Originální název

Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Carbofuran is a neurotoxic pesticide acting as an inhibitor of enzyme acetylcholinesterase (AChE). The fact that carbofuran is able to inhibit AChE can be used for its assay using an appropriate sensor. AChE was immobilized into gelatin membrane. The immobilization procedure was optimized considering temperature of drying and concentration of gelatin in mixture. Long term stability and interference of organic solvent was done as well. In the final experiment, carbofuran was assayed using the prepared biosensor. Limit of detection 5.01 10-10 mol/l (0.111 ng/ml) was achieved.

Klíčová slova

biosensor; acetylcholinesterase; pesticide; inhibitor; screen printed electrode; voltammetry; amperometry

Autoři

POHANKA, M.; FUSEK, J.; ADAM, V.; KIZEK, R.

Rok RIV

2013

Vydáno

1. 1. 2013

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

8

Číslo

1

Stát

Srbská republika

Strany od

71

Strany do

79

Strany počet

9

BibTex

@article{BUT98896,
  author="Miroslav {Pohanka} and Josef {Fusek} and Vojtěch {Adam} and René {Kizek}",
  title="Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2013",
  volume="8",
  number="1",
  pages="71--79",
  issn="1452-3981"
}