Detail publikace

Synthesis and modification of quantum dots for medical applications

Originální název

Synthesis and modification of quantum dots for medical applications

Anglický název

Synthesis and modification of quantum dots for medical applications

Jazyk

en

Originální abstrakt

There is a growing interest in using semiconductor quantum dots (QDs) as optical labels for biosensing events. The size-controlled fluorescence properties of QDs, the high fluorescence quantum yields of QDs and their stability against photobleaching makes QDs superior optical labels for multiplex analysis. In this paper, biotin-conjugated glutathione (B-GSH) was synthesized using peptide bonding of the biotin carboxy group and amino group of the glutamic acid to create an alternative coating for CdTe QDs. This type of coating combines the functionality of biotin with the fluorescent properties of quantum dots to create a specific, high affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-funcionalized glutathione-coated QDs were prepared by a facile one step reduction route using Na2TeO3 as the Te source in an aqueous environment. The synthesis parameters of this simple and rapid approach, including the reaction temperature and time, the pH of the reaction solution and the molar ratio of the GSH stabilizer to Cd2+, have considerable influence on particle sizes and photoluminescence quantum yield of the CdTe QDs. Obtained QDs were separated from the excess of the B-GSH by capillary electrophoresis employing 300 mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and laser-induced fluorescence employing Ar+ ion laser (488 nm).

Anglický abstrakt

There is a growing interest in using semiconductor quantum dots (QDs) as optical labels for biosensing events. The size-controlled fluorescence properties of QDs, the high fluorescence quantum yields of QDs and their stability against photobleaching makes QDs superior optical labels for multiplex analysis. In this paper, biotin-conjugated glutathione (B-GSH) was synthesized using peptide bonding of the biotin carboxy group and amino group of the glutamic acid to create an alternative coating for CdTe QDs. This type of coating combines the functionality of biotin with the fluorescent properties of quantum dots to create a specific, high affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-funcionalized glutathione-coated QDs were prepared by a facile one step reduction route using Na2TeO3 as the Te source in an aqueous environment. The synthesis parameters of this simple and rapid approach, including the reaction temperature and time, the pH of the reaction solution and the molar ratio of the GSH stabilizer to Cd2+, have considerable influence on particle sizes and photoluminescence quantum yield of the CdTe QDs. Obtained QDs were separated from the excess of the B-GSH by capillary electrophoresis employing 300 mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and laser-induced fluorescence employing Ar+ ion laser (488 nm).

BibTex


@inproceedings{BUT75612,
  author="Jana {Pekárková} and Markéta {Vaculovičová} and Jana {Drbohlavová} and Libor {Janů} and Vojtěch {Adam} and Jan {Prášek} and René {Kizek} and Jaromír {Hubálek}",
  title="Synthesis and modification of quantum dots for medical applications",
  annote="There is a growing interest in using semiconductor quantum dots (QDs) as optical labels for biosensing events. The size-controlled fluorescence properties of QDs, the high fluorescence quantum yields of QDs  and their stability against photobleaching makes QDs superior optical labels for multiplex analysis.  In this paper, biotin-conjugated glutathione (B-GSH) was synthesized using peptide bonding of the biotin carboxy group and amino group of the glutamic acid to create an alternative coating for CdTe QDs. This type of coating combines the functionality of biotin with the fluorescent properties of quantum dots to create a specific, high affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin.  Biotin-funcionalized glutathione-coated QDs were prepared by a facile one step reduction route using Na2TeO3 as the Te source in an aqueous environment. The synthesis parameters of this simple and rapid approach, including the reaction temperature and time, the pH of the reaction solution and the molar ratio of the GSH stabilizer to Cd2+, have considerable influence on particle sizes and photoluminescence quantum yield of the CdTe QDs. Obtained QDs were separated from the excess of the B-GSH by capillary electrophoresis employing 300 mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and laser-induced fluorescence employing Ar+ ion laser (488 nm).",
  address="TANGER spol. s r.o.",
  booktitle="NANOCON 2011 - sborník příspěvků",
  chapter="75612",
  howpublished="electronic, physical medium",
  institution="TANGER spol. s r.o.",
  year="2011",
  month="september",
  pages="1--6",
  publisher="TANGER spol. s r.o.",
  type="conference paper"
}