Detail publikace

Investigation of the antioxidant properties of metallothionein in transgenic tobacco plants using voltammetry at a carbon paste electrode

ADAM, V. PRÁŠEK, J. ZEHNÁLEK, J. HUBÁLEK, J. KIZEK, R.

Originální název

Investigation of the antioxidant properties of metallothionein in transgenic tobacco plants using voltammetry at a carbon paste electrode

Anglický název

Investigation of the antioxidant properties of metallothionein in transgenic tobacco plants using voltammetry at a carbon paste electrode

Jazyk

en

Originální abstrakt

Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals.

Anglický abstrakt

Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals.

Dokumenty

BibTex


@article{BUT72582,
  author="Violetta {Shestivska} and Vojtěch {Adam} and Jan {Prášek} and Martina {Macková} and Josef {Zehnálek} and Jaromír {Hubálek} and René {Kizek}",
  title="Investigation of the antioxidant properties of metallothionein in transgenic tobacco plants using voltammetry at a carbon paste electrode",
  annote="Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing  metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals.",
  chapter="72582",
  number="7",
  volume="6",
  year="2011",
  month="july",
  pages="2869--2883",
  type="journal article in Web of Science"
}