Detail publikace

Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium

NOVÁKOVÁ, M. KOLÁŘOVÁ, J. PROVAZNÍK, I. TÁBORSKÁ, E.

Originální název

Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium

Český název

Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium

Typ

článek v časopise - ostatní, Jost

Jazyk

cs

Originální abstrakt

Voltage-sensitive dyes (VSD's) are used in basic cardiology research to record transient potential changes in various cardiac preparations. In our laboratory, action potentials (APs) are recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining with this VSD were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff. Staining was done either by coronary perfusion with low dye concentration or with bolus injection of highly concentrated dye into the aorta. Electrogram and coronary flow were followed continuously. During the staining and washout from dye, prominent electrophysiological changes occurred such as decrease of spontaneous heart rate, partial AV block and changes of ST-T segment, accompanied with decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded putative ischemic damage of myocardium. Good viability of stained preparation was proved by electron microscopy. Since in rabbit hearts the impact of VSD was less pronounced, we conclude that rabbit myocardium is more resistant to the changes triggered by VSD application. It may be caused by different potassium channels in the membrane of cardiomyocytes in these two species. Staining with low dye concentration leads to its better persistence in the tissue.

Český abstrakt

Voltage-sensitive dyes (VSD's) are used in basic cardiology research to record transient potential changes in various cardiac preparations. In our laboratory, action potentials (APs) are recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining with this VSD were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff. Staining was done either by coronary perfusion with low dye concentration or with bolus injection of highly concentrated dye into the aorta. Electrogram and coronary flow were followed continuously. During the staining and washout from dye, prominent electrophysiological changes occurred such as decrease of spontaneous heart rate, partial AV block and changes of ST-T segment, accompanied with decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded putative ischemic damage of myocardium. Good viability of stained preparation was proved by electron microscopy. Since in rabbit hearts the impact of VSD was less pronounced, we conclude that rabbit myocardium is more resistant to the changes triggered by VSD application. It may be caused by different potassium channels in the membrane of cardiomyocytes in these two species. Staining with low dye concentration leads to its better persistence in the tissue.

Klíčová slova

di-4-ANEPPS, myocardium, rabbit, guinea pig

Rok RIV

2008

Vydáno

24.01.2008

Strany od

45

Strany do

54

Strany počet

10

Dokumenty

BibTex


@article{BUT44120,
  author="Marie {Nováková} and Jana {Kolářová} and Ivo {Provazník} and Eva {Táborská}",
  title="Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium",
  annote="Voltage-sensitive dyes (VSD's) are used in basic cardiology research to record transient potential changes in various cardiac preparations. In our laboratory, action potentials (APs) are recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining with this VSD were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff. Staining was done either by coronary perfusion with low dye concentration or with bolus injection of highly concentrated dye into the aorta. Electrogram and coronary flow were followed continuously. During the staining and washout from dye, prominent electrophysiological changes occurred such as decrease of spontaneous heart rate, partial AV block and changes of ST-T segment, accompanied with decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded putative ischemic damage of myocardium. Good viability of stained preparation was proved by electron microscopy. Since in rabbit hearts the impact of VSD was less pronounced, we conclude that rabbit myocardium is more resistant to the changes triggered by VSD application. It may be caused by different potassium channels in the membrane of cardiomyocytes in these two species. Staining with low dye concentration leads to its better persistence in the tissue.",
  chapter="44120",
  howpublished="print",
  journal="General Physiology and Biophysics",
  number="1",
  volume="26",
  year="2008",
  month="january",
  pages="45--54",
  type="journal article - other"
}