Detail publikace

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

ZHANG, H. CAO, L. BRODSKÝ, J. GABLECH, I. XU, F. LI, Z. KORABEČNÁ, M. NEUŽIL, P.

Originální název

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR (dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from pathogen detection, and cancer screening to environment monitoring. The manuscript outlines the foundational theories behind both technologies and scrutinizes their capabilities, advances, and limits. It deeply investigates the primary factors based on the sample's nature, sample preparation strategies, and employing key parameters like reliability, accuracy, and specificity, thus critically evaluating the merits and demerits of each technology. It also proposes the avenues of research and development that impact both technologies. The paper concludes that the choice between qPCR and dPCR depends on factors such as the nature of the sample, the purpose of the study, and practical considerations like cost and operational ease. Our overarching goal is to furnish a guide that aids researchers in selecting the optimal PCR-based technique for their assay development.

Klíčová slova

qPCR; dPCR

Autoři

ZHANG, H.; CAO, L.; BRODSKÝ, J.; GABLECH, I.; XU, F.; LI, Z.; KORABEČNÁ, M.; NEUŽIL, P.

Vydáno

1. 5. 2024

Nakladatel

Elsevier

Místo

UK

ISSN

0165-9936

Periodikum

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Číslo

174

Stát

Spojené království Velké Británie a Severního Irska

Strany počet

17

URL

https://www.sciencedirect.com/science/article/pii/S0165993624001584

BibTex

@article{BUT188407,
  author="ZHANG, H. and CAO, L. and BRODSKÝ, J. and GABLECH, I. and XU, F. and LI, Z. and KORABEČNÁ, M. and NEUŽIL, P.",
  title="Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications",
  journal="TRAC-TRENDS IN ANALYTICAL CHEMISTRY",
  year="2024",
  number="174",
  pages="17",
  doi="10.1016/j.trac.2024.117676",
  issn="0165-9936",
  url="https://www.sciencedirect.com/science/article/pii/S0165993624001584"
}