Detail publikace

Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

POMPEIANO VANÍČKOVÁ, L. DO, T. VEJVODOVÁ, M. HORÁK, V. HUBÁLEK, M. EMRI, G. ZEMÁNKOVÁ, K. PAVELICOVÁ, K. KŘÍŽKOVÁ, S. FALTUSOVÁ, V. POMPEIANO, A. VACULOVIČOVÁ, M. ZÍTKA, O. VACULOVIČ, T. ADAM, V.

Originální název

Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.

Klíčová slova

Melanoma; Mass spectrometry; MeLiM

Autoři

POMPEIANO VANÍČKOVÁ, L.; DO, T.; VEJVODOVÁ, M.; HORÁK, V.; HUBÁLEK, M.; EMRI, G.; ZEMÁNKOVÁ, K.; PAVELICOVÁ, K.; KŘÍŽKOVÁ, S.; FALTUSOVÁ, V.; POMPEIANO, A.; VACULOVIČOVÁ, M.; ZÍTKA, O.; VACULOVIČ, T.; ADAM, V.

Vydáno

1. 2. 2022

ISSN

0141-8130

Periodikum

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ročník

203

Číslo

1

Stát

Nizozemsko

Strany od

583

Strany do

592

Strany počet

10

URL

https://doi.org/10.1016/j.ijbiomac.2022.01.139

BibTex

@article{BUT176883,
  author="Lucie {Pompeiano Vaníčková} and Tomáš {Do} and Markéta {Vejvodová} and Vratislav {Horák} and Martin {Hubálek} and Gabriela {Emri} and Kristýna {Zemánková} and Kristýna {Pavelicová} and Soňa {Křížková} and Veronika {Faltusová} and Antonio {Pompeiano} and Markéta {Vaculovičová} and Ondřej {Zítka} and Tomáš {Vaculovič} and Vojtěch {Adam}",
  title="Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2022",
  volume="203",
  number="1",
  pages="583--592",
  doi="10.1016/j.ijbiomac.2022.01.139",
  issn="0141-8130",
  url="https://doi.org/10.1016/j.ijbiomac.2022.01.139"
}