Detail publikace

Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

FRANGU, A. ASHRAFI, A. SÝS, M. ARBNESHI, T. METELKA, R. ADAM, V. VLČEK, M. RICHTERA, L.

Originální název

Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion((R)) layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.

Klíčová slova

amperometry; ascorbic acid; berries; biosensor; multi-wall carbon nanotubes; carbon paste electrode; tyrosinase; Trolox; antioxidant capacity

Autoři

FRANGU, A.; ASHRAFI, A.; SÝS, M.; ARBNESHI, T.; METELKA, R.; ADAM, V.; VLČEK, M.; RICHTERA, L.

Vydáno

30. 4. 2020

Nakladatel

MDPI

ISSN

2076-3417

Periodikum

Applied Sciences - Basel

Ročník

10

Číslo

7

Stát

Švýcarská konfederace

Strany od

1

Strany do

13

Strany počet

13

URL

https://www.mdpi.com/2076-3417/10/7/2497

Plný text v Digitální knihovně

http://hdl.handle.net/11012/194794

BibTex

@article{BUT164381,
  author="Arbër {Frangu} and Amirmansoor {Ashrafi} and Milan {Sýs} and Tahir {Arbneshi} and Radovan {Metelka} and Vojtěch {Adam} and Milan {Vlček} and Lukáš {Richtera}",
  title="Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes",
  journal="Applied Sciences - Basel",
  year="2020",
  volume="10",
  number="7",
  pages="1--13",
  doi="10.3390/app10072497",
  issn="2076-3417",
  url="https://www.mdpi.com/2076-3417/10/7/2497"
}