Detail publikace

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

KOMPRDA, T. SLÁDEK, Z. ŠVEHLOVÁ, V. LACKOVÁ, Z. GURÁŇ, R. DO, T. WIJACKI, J. BUCHTOVÁ, M. NEUWIRTHOVÁ, J. GÁL, B. ADAM, V. ZÍTKA, O.

Originální název

The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen alpha-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semiquantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neoangiogenesis was assessed as S > P > F approximate to Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.

Klíčová slova

Docosahexaenoic acid; eicosapentaenoic acid; Schizochytrium oil; reepithelialization; MALDI MSI; collagen alpha-1(III)

Autoři

KOMPRDA, T.; SLÁDEK, Z.; ŠVEHLOVÁ, V.; LACKOVÁ, Z.; GURÁŇ, R.; DO, T.; WIJACKI, J.; BUCHTOVÁ, M.; NEUWIRTHOVÁ, J.; GÁL, B.; ADAM, V.; ZÍTKA, O.

Vydáno

1. 12. 2019

Nakladatel

University of Veterinary and Pharmaceutical Sciences in Brno

ISSN

0001-7213

Periodikum

ACTA VETERINARIA BRNO

Ročník

88

Číslo

4

Stát

Česká republika

Strany od

443

Strany do

451

Strany počet

9

URL

https://actavet.vfu.cz/88/4/0443/

Plný text v Digitální knihovně

http://hdl.handle.net/11012/184648

BibTex

@article{BUT162414,
  author="Tomáš {Komprda} and Zbyšek {Sládek} and Veronika {Švehlová} and Zuzana {Lacková} and Roman {Guráň} and Tomáš {Do} and Jan {Wijacki} and Marcela {Buchtová} and Jana {Neuwirthová} and Břetislav {Gál} and Vojtěch {Adam} and Ondřej {Zítka}",
  title="The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging",
  journal="ACTA VETERINARIA BRNO",
  year="2019",
  volume="88",
  number="4",
  pages="443--451",
  doi="10.2754/avb201988040443",
  issn="0001-7213",
  url="https://actavet.vfu.cz/88/4/0443/"
}