Detail publikace

Effect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expression

ŠUBRTOVÁ, H. ŠPLÍCHAL, Z.

Originální název

Effect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expression

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Sarcosine is extensively discussed as potential prostate cancer oncometabolite. Sarcosine dehydrogenase (SARDH) is one of the key enzymes involved in sarcosine metabolism that catalyses oxidative demethylation of sarcosine to glycine in mitochondrion. This study investigates the effects of small interfering RNA (siRNA) mediated SARDH knockdown on gene expression of sarcosine metabolism related enzymes: glycine N-methyltransferase (GNMT), peroxisomal sarcosine oxidase (PIPOX) and dimethylglycine dehydrogenase (DMGDH). The effect of SARDH knockdown was studied in three human prostate cell lines (PNT1A, DU-145, PC3) and gene expression was evaluated by quantitative real-time PCR (qPCR). Although the lowest SARDH knockdown was achieved in cancer DU-145 cell line, the highest changes in expression of other sarcosine metabolism genes were detected. In particular, the significant increase in DMGDH and PIPOX expression was observed. Our findings revealed potential stimulation effect of SARDH knockdown on DMGDH and PIPOX expression in prostate cancer cell line DU-145.

Klíčová slova

knock-down; prostate cancer; sarcosine dehydrogenase; sarcosine metabolism

Autoři

ŠUBRTOVÁ, H.; ŠPLÍCHAL, Z.

Vydáno

31. 12. 2019

Nakladatel

Mendel University in Brno

ISBN

978-80-7509-688-3

Kniha

MendelNet 2019 Proceedings of 26th International PhD Students Conference

Strany od

648

Strany do

653

Strany počet

6

URL

https://mendelnet.cz/artkey/mnt-201901-0125_Effect-of-sarcosine-dehydrogenase-knockdown-on-sarcosine-metabolism-related-genes-expression.php?back=/magno/mnt/2019/mn1.php?secid=4

Plný text v Digitální knihovně

http://hdl.handle.net/11012/184135

BibTex

@inproceedings{BUT161420,
  author="Hana {Šubrtová} and Zbyněk {Šplíchal}",
  title="Effect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expression",
  booktitle="MendelNet 2019 Proceedings of 26th International PhD Students Conference",
  year="2019",
  pages="648--653",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-688-3",
  url="https://mendelnet.cz/artkey/mnt-201901-0125_Effect-of-sarcosine-dehydrogenase-knockdown-on-sarcosine-metabolism-related-genes-expression.php?back=/magno/mnt/2019/mn1.php?secid=4"
}