Detail publikace

Molecular imprinting technology for targeted analysis of proteins

HUTAŘOVÁ, J. VANĚČKOVÁ, T. VACULOVIČOVÁ, M. ADAM, V.

Originální název

Molecular imprinting technology for targeted analysis of proteins

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Molecular imprinting has appeared to be an effective technique for creating of selective recognition sites in synthetic polymers. This procedure comprises polymerization of monomer in a presence of target molecules (template). The subsequent template removal forms tailor-made cavities that are complementary in shape and size to the template molecules. For protein imprinting, the choice of the suitable polymers is limited and polymerization conditions need to be optimized. In our work, dopamine monomer was chosen for polymer formation due to its nontoxicity, ease of preparation and self-assembly. For the optimization of conditions, lysozyme with molecular weight 14.3 kDa was used and the functionality was evaluated by fluorimetry. Different concentration of dopamine and lysozyme for polymerization were tested. Under the optimized conditions, the limit of detection for lysozyme was found to be 7.8 µg/ml. Moreover, conditions for polymer formation for a purpose to reduce the overall time of analysis were investigated. The use of dopamine as a monomer in molecular imprinting shown to be beneficial in many aspects.

Klíčová slova

Polydopamine; molecularly imprinted polymer; lysozyme

Autoři

HUTAŘOVÁ, J.; VANĚČKOVÁ, T.; VACULOVIČOVÁ, M.; ADAM, V.

Vydáno

31. 12. 2017

Nakladatel

Mendel University in Brno

ISBN

978-80-7509-529-9

Kniha

MendelNet 2017

Strany od

873

Strany do

877

Strany počet

5

URL

https://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4

Plný text v Digitální knihovně

http://hdl.handle.net/11012/84189

BibTex

@inproceedings{BUT148729,
  author="Jitka {Hutařová} and Tereza {Vaněčková} and Markéta {Vaculovičová} and Vojtěch {Adam}",
  title="Molecular imprinting technology for targeted analysis of proteins",
  booktitle="MendelNet 2017",
  year="2017",
  pages="873--877",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-529-9",
  url="https://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4"
}