Detail publikace

Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates

VOBĚRKOVÁ, S. SOLČÁNY, V. VRŠANSKÁ, M. ADAM, V.

Originální název

Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Ligninolytic enzymes from white-rot fungi are widely used in biotechnological processes. However, the application of these enzymes as free enzymes is limited due to their instability and lack of reusability. Enzyme stabilization is therefore a major challenge in biocatalytic process research, and immobilization methods are desirable. Using cross-linked enzyme aggregates (CLEAs) such as magnetic CLEAs, porous-CLEAs and combi-CLEAs is a promising technique for overcoming these issues. Cross-linking methods can stabilize and immobilize enzymes by interconnecting enzyme molecules via multiple bonds using cross linking agents such as glutaraldehyde. The high catalyst density and microporous assembly of CLEAs guarantee high catalyst activity, which, together with their long shelf life, operational stability, and reusability, provide a cost-efficient alternative to matrix-assisted immobilization approaches. Here, we review current progress in ligninolytic enzyme immobilization and provide a comprehensive review of CLEAs. Moreover, we summarize the use of these CLEAs for biocatalysis processes, bioremediation such as dye decolourization, wastewater treatment or pharmaceutically active compound elimination.

Klíčová slova

White-rot fungi; Ligninolytic enzymes; Cross-linked enzyme aggregates; Immobilization

Autoři

VOBĚRKOVÁ, S.; SOLČÁNY, V.; VRŠANSKÁ, M.; ADAM, V.

Vydáno

1. 7. 2018

ISSN

0045-6535

Periodikum

CHEMOSPHERE

Ročník

202

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

694

Strany do

707

Strany počet

14

BibTex

@article{BUT147398,
  author="Stanislava {Voběrková} and Veronika {Solčány} and Martina {Vršanská} and Vojtěch {Adam}",
  title="Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates",
  journal="CHEMOSPHERE",
  year="2018",
  volume="202",
  number="1",
  pages="694--707",
  doi="10.1016/j.chemosphere.2018.03.088",
  issn="0045-6535"
}