Detail publikace

A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin

HEGER, Z. CERNEI, N. KUDR, J. GUMULEC, J. BLAŽKOVÁ, I. ZÍTKA, O. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V. KIZEK, R.

Originální název

A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Doxorubicin is a commonly used antineoplastic agent in the treatment of many types of cancer. Little is known about the interactions of doxorubicin with cardiac biomolecules. Serious cardiotoxicity including dilated cardiomyopathy often resulting in a fatal congestive heart failure may occur as a consequence of chemotherapy with doxorubicin. The purpose of this study was to determine the effect of exposure to doxorubicin on the changes in major amino acids in tissue of cardiac muscle (proline, taurine, glutamic acid, arginine, aspartic acid, leucine, glycine, valine, alanine, isoleucine, threonine, lysine and serine). An in vitro interaction study was performed as a comparison of amino acid profiles in heart tissue before and after application of doxorubicin. We found that doxorubicin directly influences myocardial amino acid representation even at low concentrations. In addition, we performed an interaction study that resulted in the determination of breaking points for each of analyzed amino acids. Lysine, arginine, -alanine, valine and serine were determined as the most sensitive amino acids. Additionally we compared amino acid profiles of myocardium before and after exposure to doxorubicin. The amount of amino acids after interaction with doxorubicin was significantly reduced (p = 0.05). This fact points at an ability of doxorubicin to induce changes in quantitative composition of amino acids in myocardium. Moreover, this confirms that the interactions between doxorubicin and amino acids may act as another factor most likely responsible for adverse effects of doxorubicin on myocardium.

Klíčová slova

myocardium; cardiomyopathy; interaction; amide bond; spectrophotometry; ion-exchange liquid chromatography

Autoři

HEGER, Z.; CERNEI, N.; KUDR, J.; GUMULEC, J.; BLAŽKOVÁ, I.; ZÍTKA, O.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.; KIZEK, R.

Vydáno

1. 11. 2013

Nakladatel

MDPI

ISSN

1422-0067

Periodikum

International Journal of Molecular Sciences

Ročník

14

Číslo

11

Stát

Švýcarská konfederace

Strany od

21629

Strany do

21646

Strany počet

18

URL

https://www.mdpi.com/1422-0067/14/11/21629

Plný text v Digitální knihovně

http://hdl.handle.net/11012/137222

BibTex

@article{BUT145130,
  author="Zbyněk {Heger} and Natalia Vladimirovna {Cernei} and Jiří {Kudr} and Jaromír {Gumulec} and Iva {Blažková} and Ondřej {Zítka} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam} and René {Kizek}",
  title="A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin",
  journal="International Journal of Molecular Sciences",
  year="2013",
  volume="14",
  number="11",
  pages="21629--21646",
  doi="10.3390/ijms141121629",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/14/11/21629"
}