Detail publikace

An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents

POHANKA, M. ADAM, V. KIZEK, R.

Originální název

An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10(-12) mol/L for sarin, 6.31 x 10(-12) mol/L for soman, 6.17 x 10(-11) mol/L for tabun, and 2.19 x 10(-11) mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples.

Klíčová slova

biosensor; acetylcholinesterase; sarin; tabun; soman; VX; inhibitor; screen printed electrode; voltammetry; amperometry

Autoři

POHANKA, M.; ADAM, V.; KIZEK, R.

Vydáno

1. 9. 2013

Nakladatel

MDPI

ISSN

1424-8220

Periodikum

SENSORS

Ročník

13

Číslo

9

Stát

Švýcarská konfederace

Strany od

11498

Strany do

11506

Strany počet

7

URL

https://www.mdpi.com/1424-8220/13/9/11498

Plný text v Digitální knihovně

http://hdl.handle.net/11012/137241

BibTex

@article{BUT143113,
  author="Miroslav {Pohanka} and Vojtěch {Adam} and René {Kizek}",
  title="An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents",
  journal="SENSORS",
  year="2013",
  volume="13",
  number="9",
  pages="11498--11506",
  doi="10.3390/s130911498",
  issn="1424-8220",
  url="https://www.mdpi.com/1424-8220/13/9/11498"
}