Detail publikace

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

GURÁŇ, R. POMPEIANO VANÍČKOVÁ, L. HORÁK, V. KŘÍŽKOVÁ, S. MICHÁLEK, P. HEGER, Z. ZÍTKA, O. ADAM, V.

Originální název

MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background Treatment of advanced cutaneous melanoma remains challenging, and new data on melanoma biology are required. The most widely accepted criteria for the prognostic evaluation of melanoma are histopathological and clinical parameters, and the identification of additional tumor markers is thus of paramount importance. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI), an important tool in cancer research, is useful for unraveling the molecular profile of melanoma. Methodology/Principal findings In this report, we used the melanoma-bearing Libechov minipig (MeLiM), a unique animal model that allows observation of the complete spontaneous regression of invasive cutaneous melanoma, to investigate i) the differences between melanoma and healthy skin protein profiles and ii) the proteins potentially involved in spontaneous regression. The MeLiM tissues were cryosected, histologically characterized, analyzed by MALDI MSI, and immunohistologically stained. Multivariate statistical analyses of the MALDI MSI data revealed ten relevant m/z ions, of which the expression levels varied significantly among the studied MeLiM tissues. These ion peaks were used to create mass ion images/maps and visualize the differences between tumor and healthy skin specimens, as well as among histologically characterized tissue regions. Conclusions/Significance Protein profiles comprising ten statistically significant mass ion peaks useful for differentiating cutaneous melanoma and healthy skin tissues were determined. Peaks at m/z 3044, 6011, 6140 and 10180 were overexpressed in melanoma compared with healthy skin tissue. More specifically, m/z 6140 was expressed at significantly (p < 0.05) higher levels in normally growing melanoma regions than in regions with early and late spontaneous regression. This study demonstrates the clinical utility of MALDI MSI for the analysis of tissue cryosections at a molecular level.

Klíčová slova

protein profiles; MALDI MSI; MeLiM melanoma

Autoři

GURÁŇ, R.; POMPEIANO VANÍČKOVÁ, L.; HORÁK, V.; KŘÍŽKOVÁ, S.; MICHÁLEK, P.; HEGER, Z.; ZÍTKA, O.; ADAM, V.

Vydáno

8. 12. 2017

Nakladatel

PLOS

ISSN

1932-6203

Periodikum

PLOS ONE

Ročník

12

Číslo

12

Stát

Spojené státy americké

Strany od

1

Strany do

15

Strany počet

15

URL

http://europepmc.org/articles/PMC5722329?pdf=render

Plný text v Digitální knihovně

http://hdl.handle.net/11012/137971

BibTex

@article{BUT142853,
  author="Roman {Guráň} and Lucie {Pompeiano Vaníčková} and Vratislav {Horák} and Soňa {Křížková} and Petr {Michálek} and Zbyněk {Heger} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles
",
  journal="PLOS ONE",
  year="2017",
  volume="12",
  number="12",
  pages="1--15",
  doi="10.1371/journal.pone.0189305",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC5722329?pdf=render"
}