Detail publikace

An enzymatic assay based on luciferase Ebola virus-like particles for evaluation of virolytic activity of antimicrobial peptides

PEŠKOVÁ, M. HEGER, Z. JANDA, P. ADAM, V. PEKAŘÍK, V.

Originální název

An enzymatic assay based on luciferase Ebola virus-like particles for evaluation of virolytic activity of antimicrobial peptides

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Antimicrobial peptides are currently considered as promising antiviral compounds. Current assays to evaluate the effectivity of peptides against enveloped viruses based on liposomes or hemolysis are encumbered by the artificial nature of liposomes or distinctive membrane composition of used erythrocytes. We propose a novel assay system based on enzymatic Ebola virus-like particles containing sensitive luciferase reporter. The assay was validated with several cationic and anionic peptides and compared with lentivirus inactivation and hemolytic assays. The assay is sensitive and easy to perform in standard biosafety level laboratory with potential for high-throughput screens. The use of virus-like particles in the assay provides a system as closely related to the native viruses as possible eliminating some issues associated with other more artificial set ups. We have identified CAM-W (KWKLWKKIEKWGQGIGAVLKWLTTWL) as a peptide with the greatest antiviral activity against infectious lentiviral vectors and filoviral virus-like particles.

Klíčová slova

Virus-like particles; Cytotoxic peptides; Antimicrobial peptides; Nanoluciferase; Hemolytic activity; Virolytic activity; Lentivirus; Ebola; Marburg

Autoři

PEŠKOVÁ, M.; HEGER, Z.; JANDA, P.; ADAM, V.; PEKAŘÍK, V.

Vydáno

1. 2. 2017

ISSN

0196-9781

Periodikum

PEPTIDES

Ročník

88

Číslo

1

Stát

Spojené státy americké

Strany od

87

Strany do

96

Strany počet

10

BibTex

@article{BUT134365,
  author="PEŠKOVÁ, M. and HEGER, Z. and JANDA, P. and ADAM, V. and PEKAŘÍK, V.",
  title="An enzymatic assay based on luciferase Ebola virus-like particles for evaluation of virolytic activity of antimicrobial peptides",
  journal="PEPTIDES",
  year="2017",
  volume="88",
  number="1",
  pages="87--96",
  doi="10.1016/j.peptides.2016.12.015",
  issn="0196-9781"
}