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VANĚČKOVÁ, T. MILOSAVLJEVIĆ, V. KRYŠTOFOVÁ, O. ADAM, V. VACULOVIČOVÁ, M.
Originální název
Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Nanoparticle visualization has extensive potential for their utilization in targeted drug delivery tracking through wide range of biological environments. In this regard, quantum dot nanoparticles may represent a suitable option as they are characterized by very good fluorescent properties. This study is focused on application of quantum dot nanoparticles for fluorescence imaging of plants. A series of in vivo experiments was carried out on leaves of sunflower plant (Helianthus annuus). Leaves were immersed in water solution of CdTe and CdTe/ZnS quantum dots and were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, Massachusets, USA). Captured images were further processed and the intensity of fluorescence analyzed by Bruker Molecular Imaging Software. Fluorescence imaging of plants encounters difficulties due to autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophyll. However, this can be suppressed by using adequate excitation and emission filters for fluorescence images acquisition. Moreover, visualization of nanoparticles in plants can be enhanced by multispectral imaging and spectral modeling for autofluorescence unmixing. In this study, evaluation regarding optimal characteristics and modifications of quantum dot nanoparticles for fluorescence imaging in plants is provided as well as recommendations on image acquisition setting and further image postprocessing.
Klíčová slova
QDs; in VIVO; fluorescence; nanoparticles
Autoři
VANĚČKOVÁ, T.; MILOSAVLJEVIĆ, V.; KRYŠTOFOVÁ, O.; ADAM, V.; VACULOVIČOVÁ, M.
Vydáno
21. 10. 2016
Nakladatel
AMOS repro Ltd.
Místo
Ostrava, Czech Republic
ISBN
978-80-87294-68-0
Kniha
NANOCON 2016
Strany od
112
Strany do
Strany počet
1
BibTex
@misc{BUT133444, author="Tereza {Vaněčková} and Vedran {Milosavljević} and Olga {Kryštofová} and Vojtěch {Adam} and Markéta {Vaculovičová}", title="Monitoring of quantum dot distribution in plants by in vivo fluorescence imaging", booktitle="NANOCON 2016", year="2016", pages="112--112", publisher="AMOS repro Ltd.", address="Ostrava, Czech Republic", isbn="978-80-87294-68-0", note="abstract" }