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ANÝZ, J. VYSLOUŽILOVÁ, L. VACULOVIČ, T. TVRDOŇOVÁ, M. KANICKÝ, V. HAASE, H. HORÁK, V. ŠTĚPÁNKOVÁ, O. HEGER, Z. ADAM, V.
Originální název
Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.
Klíčová slova
LA-ICP-MS; BEARING LIBECHOV MINIPIG; MIXED-EFFECTS MODELS; METALLOTHIONEIN-OVEREXPRESSION; MELANOMA PATIENTS; ZINC LEVELS; COPPER; MELANOBLASTOMA; ELEMENTS; STRESS
Autoři
ANÝZ, J.; VYSLOUŽILOVÁ, L.; VACULOVIČ, T.; TVRDOŇOVÁ, M.; KANICKÝ, V.; HAASE, H.; HORÁK, V.; ŠTĚPÁNKOVÁ, O.; HEGER, Z.; ADAM, V.
Vydáno
10. 1. 2017
Nakladatel
Springer Nature
ISSN
2045-2322
Periodikum
Scientific Reports
Ročník
7
Číslo
40169
Stát
Spojené království Velké Británie a Severního Irska
Strany od
40169-1
Strany do
40169-13
Strany počet
13
URL
https://www.nature.com/articles/srep40169
Plný text v Digitální knihovně
http://hdl.handle.net/11012/69381