Detail publikace

Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application

STANISAVLJEVIC, M. KŘÍŽKOVÁ, S. VACULOVIČOVÁ, M. KIZEK, R. ADAM, V.

Originální název

Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Fluorescence resonance energy transfer (FRET)-based nanosensors with quantum dots (QDs) as donors and organic dyes as acceptors have long been of interest for the detection of biomolecules such as nucleic acids, but their low FRET efficiency in bulk solution has prevented the sensitive detection of nucleic acids due to the large size of the QDs and the long length of nucleic acids. Here we describe a novel approach to improve the detection sensitivity of QD-based nanosensors using single-molecule detection in a capillary flow. In comparison with bulk measurement, single-molecule detection in a capillary flow possesses the unique advantages of improved FRET efficiency, high sensitivity, prevention of photobleaching, and low sample consumption. Greater FRET efficiency was obtained due to the deformation of DNA in the capillary stream. This technique can be easily extended to sensitive bimolecular analysis in microfluidic chips, and it may also offer a promising approach to study the deformation of small nucleic acids in fluid flow.

Klíčová slova

Quantum dots, Fluorescence resonanceenergytransfer, Sensor, Nucleicacid,Enzyme

Autoři

STANISAVLJEVIC, M.; KŘÍŽKOVÁ, S.; VACULOVIČOVÁ, M.; KIZEK, R.; ADAM, V.

Rok RIV

2015

Vydáno

15. 12. 2015

ISSN

0956-5663

Periodikum

BIOSENSORS & BIOELECTRONICS

Ročník

74

Číslo

1

Stát

Nizozemsko

Strany od

562

Strany do

574

Strany počet

13

BibTex

@article{BUT121157,
  author="Maja {Stanisavljevic} and Soňa {Křížková} and Markéta {Vaculovičová} and René {Kizek} and Vojtěch {Adam}",
  title="Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2015",
  volume="74",
  number="1",
  pages="562--574",
  doi="10.1016/j.bios.2015.06.076",
  issn="0956-5663"
}