Detail publikace

Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes

MERLOS RODRIGO, M. KREJČOVÁ, L. HEGER, Z. ZÍTKA, O. ADAM, V. KIZEK, R.

Originální název

Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Influenza viruses, as etiological agent of acute respiratory-related diseases, cause seasonal epidemics and/or less frequently pandemics with substantial mortality across the world. They display high mutation rates and complex evolutionary patterns inducing often significant resistance to antiviral drugs. We considered an electrochemical detection method, so called CombiMatrix ElectraSense core technology, to create one influenza array containing probes of all genes encodable by different subtypes of influenza type A genome. We detected particularly: neuraminidase, hemagglutinin, nucleocapsid protein, matrix protein 1, matrix protein 2, polymerase PB1, polymerase PA and nuclear export protein. Moreover, we succeeded to detect in vitro prepared mutations of the mentioned genes and it was found that the suggested array might be a suitable method not only for detection of the expression of the genes but also for detection of their mutations

Klíčová slova

Influenza Virus; Microarray; Mutation Sequence; Antiviral Drug; Electrochemistry

Autoři

MERLOS RODRIGO, M.; KREJČOVÁ, L.; HEGER, Z.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

Rok RIV

2015

Vydáno

1. 12. 2015

Nakladatel

ESG

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

10

Číslo

12

Stát

Srbská republika

Strany od

9952

Strany do

9967

Strany počet

16

URL

http://www.electrochemsci.org/papers/vol10/101209952.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/11012/201454

BibTex

@article{BUT120440,
  author="Miguel Ángel {Merlos Rodrigo} and Ludmila {Krejčová} and Zbyněk {Heger} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="12",
  pages="9952--9967",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/101209952.pdf"
}