Detail publikace

Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Originální název

Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Anglický název

Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Jazyk

en

Originální abstrakt

Physiological responses of Scenedesmus quadricauda to Cr(VI) and Cr(III) excess were studied in buffer with circumneutral pH (6.5). Total Cr content was similar in low (1 lM of both oxidation states) but higher in 10 lM Cr(VI) treatment and high accumulation potential was detected (80–82% and 41–65% in 1 and 10 lM treatments, respectively). Specific fluorescence indicator (6-((anthracen-9-yl) methyleneamino)- 2H-chromen-2-one) confirmed partial reduction of Cr(VI) to Cr(III) under exposure conditions. Viability and chlorophyll autofluorescence were more depleted by Cr(VI) while Cr(III) stimulated increase in ROS and lipid peroxidation. Antioxidative enzyme activities showed significantly higher values in 10 lM treatments of both Cr oxidation states. Depletion of mitochondrial proteins was not reflected in alteration of total soluble proteins indicating sensitivity of this organelle to Cr and TTC test showed no clear oxidation state-related effect. In this view, Cr(VI) is not more toxic than Cr(III) at least for some parameters. Subsequent study with the application of 10 lM Cr(VI) confirmed that HEPES buffer is more suitable exposure solution for toxicological studied than water or inorganic salts (higher chlorophyll autofluorescence was observed) and pH 6.5 is more suitable than low or high pH (4.5 or 8.5) in terms of Cr uptake. Another known Cr(III) fluorescence indicator (naphthalimide–rhodamine) also confirmed partial reduction of Cr(VI) to Cr(III) at acidic pH but only traces were seen at alkaline pH.

Anglický abstrakt

Physiological responses of Scenedesmus quadricauda to Cr(VI) and Cr(III) excess were studied in buffer with circumneutral pH (6.5). Total Cr content was similar in low (1 lM of both oxidation states) but higher in 10 lM Cr(VI) treatment and high accumulation potential was detected (80–82% and 41–65% in 1 and 10 lM treatments, respectively). Specific fluorescence indicator (6-((anthracen-9-yl) methyleneamino)- 2H-chromen-2-one) confirmed partial reduction of Cr(VI) to Cr(III) under exposure conditions. Viability and chlorophyll autofluorescence were more depleted by Cr(VI) while Cr(III) stimulated increase in ROS and lipid peroxidation. Antioxidative enzyme activities showed significantly higher values in 10 lM treatments of both Cr oxidation states. Depletion of mitochondrial proteins was not reflected in alteration of total soluble proteins indicating sensitivity of this organelle to Cr and TTC test showed no clear oxidation state-related effect. In this view, Cr(VI) is not more toxic than Cr(III) at least for some parameters. Subsequent study with the application of 10 lM Cr(VI) confirmed that HEPES buffer is more suitable exposure solution for toxicological studied than water or inorganic salts (higher chlorophyll autofluorescence was observed) and pH 6.5 is more suitable than low or high pH (4.5 or 8.5) in terms of Cr uptake. Another known Cr(III) fluorescence indicator (naphthalimide–rhodamine) also confirmed partial reduction of Cr(VI) to Cr(III) at acidic pH but only traces were seen at alkaline pH.

BibTex


@article{BUT108282,
  author="Jozef {Kováčik} and Petr {Babula} and Pavel {Hedbavny} and Olga {Kryštofová} and Ivo {Provazník}",
  title="Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object",
  annote="Physiological responses of Scenedesmus quadricauda to Cr(VI) and Cr(III) excess were studied in buffer
with circumneutral pH (6.5). Total Cr content was similar in low (1 lM of both oxidation states) but
higher in 10 lM Cr(VI) treatment and high accumulation potential was detected (80–82% and 41–65%
in 1 and 10 lM treatments, respectively). Specific fluorescence indicator (6-((anthracen-9-yl) methyleneamino)-
2H-chromen-2-one) confirmed partial reduction of Cr(VI) to Cr(III) under exposure conditions.
Viability and chlorophyll autofluorescence were more depleted by Cr(VI) while Cr(III) stimulated increase
in ROS and lipid peroxidation. Antioxidative enzyme activities showed significantly higher values in
10 lM treatments of both Cr oxidation states. Depletion of mitochondrial proteins was not reflected in
alteration of total soluble proteins indicating sensitivity of this organelle to Cr and TTC test showed no
clear oxidation state-related effect. In this view, Cr(VI) is not more toxic than Cr(III) at least for some
parameters. Subsequent study with the application of 10 lM Cr(VI) confirmed that HEPES buffer is more
suitable exposure solution for toxicological studied than water or inorganic salts (higher chlorophyll
autofluorescence was observed) and pH 6.5 is more suitable than low or high pH (4.5 or 8.5) in terms
of Cr uptake. Another known Cr(III) fluorescence indicator (naphthalimide–rhodamine) also confirmed
partial reduction of Cr(VI) to Cr(III) at acidic pH but only traces were seen at alkaline pH.",
  chapter="108282",
  doi="10.1016/j.chemosphere.2014.05.074",
  number="120",
  volume="2015",
  year="2014",
  month="june",
  pages="23--30",
  type="journal article"
}