Detail publikace

Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

MERLOS RODRIGO, M. CERNEI, N. KOMÍNKOVÁ, M. ZÍTKA, O. BEKLOVÁ, M. ZEHNÁLEK, J. KIZEK, R. ADAM, V.

Originální název

Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes

Klíčová slova

ion exchange chromatography; mass spectrometry; MALDI-TOF; phytochelatin; cadmium; intramolecular complex

Autoři

MERLOS RODRIGO, M.; CERNEI, N.; KOMÍNKOVÁ, M.; ZÍTKA, O.; BEKLOVÁ, M.; ZEHNÁLEK, J.; KIZEK, R.; ADAM, V.

Rok RIV

2013

Vydáno

28. 3. 2013

Nakladatel

MDPI

ISSN

1660-4601

Periodikum

International Journal of Environmental Research and Public Health

Ročník

10

Číslo

4

Stát

Švýcarská konfederace

Strany od

1304

Strany do

1311

Strany počet

8

URL

http://www.mdpi.com/1660-4601/10/4/1304

Plný text v Digitální knihovně

http://hdl.handle.net/11012/70096

BibTex

@article{BUT101373,
  author="Miguel Ángel {Merlos Rodrigo} and Natalia Vladimirovna {Cernei} and Markéta {Komínková} and Ondřej {Zítka} and Miroslava {Beklová} and Josef {Zehnálek} and René {Kizek} and Vojtěch {Adam}",
  title="Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes",
  journal="International Journal of Environmental Research and Public Health",
  year="2013",
  volume="10",
  number="4",
  pages="1304--1311",
  doi="10.3390/ijerph10041304",
  issn="1660-4601",
  url="http://www.mdpi.com/1660-4601/10/4/1304"
}