Detail publikace

Improvement of Electrophoresis Performance by Spectral Analysis

Originální název

Improvement of Electrophoresis Performance by Spectral Analysis

Anglický název

Improvement of Electrophoresis Performance by Spectral Analysis

Jazyk

en

Originální abstrakt

The paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using real-time spectral analysis of the samples to increase its performance. A laser beam illuminates the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.

Anglický abstrakt

The paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using real-time spectral analysis of the samples to increase its performance. A laser beam illuminates the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.

BibTex


@article{BUT90646,
  author="Layal {Abo Khayal} and René {Kizek} and Ivo {Provazník}",
  title="Improvement of Electrophoresis Performance by Spectral Analysis",
  annote="The paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using real-time spectral analysis of the samples to increase its performance. A laser beam illuminates the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.",
  chapter="90646",
  number="51",
  volume="11",
  year="2012",
  month="april",
  pages="11329--11332",
  type="journal article - other"
}