Detail publikace

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

HÚSKA, D. ADAM, V. BABULA, P. TRNKOVÁ, L. HUBÁLEK, J. ZEHNÁLEK, J. HAVEL, L. KIZEK, R.

Originální název

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Klíčová slova

Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles

Autoři

HÚSKA, D.; ADAM, V.; BABULA, P.; TRNKOVÁ, L.; HUBÁLEK, J.; ZEHNÁLEK, J.; HAVEL, L.; KIZEK, R.

Rok RIV

2011

Vydáno

1. 4. 2011

Nakladatel

SPRINGER WIEN,

ISSN

0026-3672

Periodikum

Microchimica Acta

Ročník

2012 (173)

Číslo

1-2

Stát

Rakouská republika

Strany od

189

Strany do

197

Strany počet

9

BibTex

@article{BUT75745,
  author="HÚSKA, D. and ADAM, V. and BABULA, P. and TRNKOVÁ, L. and HUBÁLEK, J. and ZEHNÁLEK, J. and HAVEL, L. and KIZEK, R.",
  title="Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA",
  journal="Microchimica Acta",
  year="2011",
  volume="2012 (173)",
  number="1-2",
  pages="189--197",
  issn="0026-3672"
}