Detail publikace

Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots

Originální název

Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots

Anglický název

Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots

Jazyk

en

Originální abstrakt

This paper is focused on the investigation of conjugate nanostructures consisting of superparamagnetic nanoparticles (NPs) and CdSe/CdS quantum dots (QDs) with core/shell structure. In the first step, we prepared streptavidin (STP) conjugated Fe2O3 magnetic particles. The next stage was preparation of QDs using method based on surface modification of hydrophobic core/shell CdSe/CdS with biotinylated glutathione. Glutathione (GSH) belongs to the most abundant peptides in organisms maintaining redox status, which, from the particle point of view, makes the QDs water-soluble and stabilized in aqueous solution. Biotin molecule is known for its excellent affinity to streptavidin and hence allows the conjugation of both nanostructures (magnetic and light-emitting). We tested the influence of both prepared nanostructures on growth and viability of fibroblasts and BY-2 tobacco cells, respectively. NPs-STP and QDs-GSH may not pose a threat to cells, but non-modified nanoparticles and QDs without GSH had a very adverse effect on cells.

Anglický abstrakt

This paper is focused on the investigation of conjugate nanostructures consisting of superparamagnetic nanoparticles (NPs) and CdSe/CdS quantum dots (QDs) with core/shell structure. In the first step, we prepared streptavidin (STP) conjugated Fe2O3 magnetic particles. The next stage was preparation of QDs using method based on surface modification of hydrophobic core/shell CdSe/CdS with biotinylated glutathione. Glutathione (GSH) belongs to the most abundant peptides in organisms maintaining redox status, which, from the particle point of view, makes the QDs water-soluble and stabilized in aqueous solution. Biotin molecule is known for its excellent affinity to streptavidin and hence allows the conjugation of both nanostructures (magnetic and light-emitting). We tested the influence of both prepared nanostructures on growth and viability of fibroblasts and BY-2 tobacco cells, respectively. NPs-STP and QDs-GSH may not pose a threat to cells, but non-modified nanoparticles and QDs without GSH had a very adverse effect on cells.

BibTex


@article{BUT50993,
  author="Jana {Pekárková} and Jana {Drbohlavová} and Petr {Babula} and Vojtěch {Adam} and Jaromír {Hubálek} and Ivo {Provazník} and René {Kizek}",
  title="Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots",
  annote="This paper is focused on the investigation of conjugate nanostructures consisting of superparamagnetic  nanoparticles (NPs) and CdSe/CdS quantum dots (QDs) with core/shell structure. In the first step, we prepared streptavidin (STP) conjugated Fe2O3 magnetic particles. The next stage was preparation of QDs using method based on surface modification of hydrophobic core/shell CdSe/CdS with biotinylated glutathione. Glutathione (GSH) belongs to the most abundant peptides in organisms maintaining redox status, which, from the particle point of view, makes the QDs water-soluble and stabilized in aqueous solution. Biotin molecule is known for its excellent affinity to streptavidin and hence allows the conjugation of both nanostructures (magnetic and light-emitting). We tested the influence of both prepared nanostructures on growth and viability of fibroblasts and BY-2 tobacco cells, respectively. NPs-STP and QDs-GSH may not pose a threat to cells, but non-modified nanoparticles and QDs without GSH had a very adverse effect on cells.",
  address="Elsevier Ltd",
  chapter="50993",
  institution="Elsevier Ltd",
  number="5",
  volume="2010",
  year="2010",
  month="september",
  pages="922--925",
  publisher="Elsevier Ltd",
  type="journal article - other"
}