Detail publikace

Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

Originální název

Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

Anglický název

Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

Jazyk

en

Originální abstrakt

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Anglický abstrakt

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

BibTex


@article{BUT48191,
  author="Jana {Kolářová} and Kateřina {Fialová} and Oto {Janoušek} and Marie {Nováková} and Ivo {Provazník}",
  title="Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart",
  annote="Monophasic action potential (MAP) can be recorded from the heart surface by optical method
based on fluorescence measurement. The motion of isolated heart during experiment caused
additional noise in recorded signal. The motion artifact can be eliminated by ratiometric
fluorescence emission measurements. This study is based on experiments in which optical
MAP measurement is done by single-wavelength and dual-wavelength measurement of
fluorescence emission. Both recording setups are presented and their advantages and
disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts
perfused according to Langendorff are presented. Simultaneous electrograms (EG) and
MAPs recording are analyzed and measurement of velocity of impulse conduction through
heart tissue is presented.",
  address="AV ČR",
  chapter="48191",
  institution="AV ČR",
  journal="Physiological Research",
  number="Suppl 1",
  volume="59",
  year="2010",
  month="may",
  pages="S71--S80",
  publisher="AV ČR",
  type="journal article in Web of Science"
}