Detail publikace

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

Originální název

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

Anglický název

Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart

Jazyk

en

Originální abstrakt

Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.

Anglický abstrakt

Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.

BibTex


@inproceedings{BUT35735,
  author="Kateřina {Fialová} and Jana {Kolářová} and Jiří {Sekora} and Milan {Tannenberg} and Marie {Nováková} and Ivo {Provazník}",
  title="Electrophysiological effects of Voltage-Sensitive Dye di-4-ANEPPS in Rabbit and Guinea Pig Isolated Heart",
  annote="Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.",
  address="Brno University of Technology",
  booktitle="Proceedings of Biosignal 2010: Analysis of Biomedical Signals and Images",
  chapter="35735",
  howpublished="print",
  institution="Brno University of Technology",
  year="2010",
  month="june",
  pages="108--113",
  publisher="Brno University of Technology",
  type="conference paper"
}