Detail publikace

PCR past, present and future

ZHU, H. ZHANG, H. XU, Y. LAŠŠÁKOVÁ, S. KORABEČNÁ, M. NEUŽIL, P.

Originální název

PCR past, present and future

Anglický název

PCR past, present and future

Jazyk

en

Originální abstrakt

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Anglický abstrakt

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

Dokumenty

BibTex


@article{BUT165710,
  author="Pavel {Neužil}",
  title="PCR past, present and future",
  annote="PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.",
  address="FUTURE SCI LTD",
  chapter="165710",
  doi="10.2144/btn-2020-0057",
  howpublished="online",
  institution="FUTURE SCI LTD",
  number="4",
  volume="69",
  year="2020",
  month="august",
  pages="1--10",
  publisher="FUTURE SCI LTD",
  type="journal article in Web of Science"
}