Detail publikace

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Zhu, HL. Zhang, HQ. Ni, S. Korabecna, M. Yobas, L. Neuzil, P.

Originální název

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Anglický název

The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond

Jazyk

en

Originální abstrakt

Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.

Anglický abstrakt

Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.

Dokumenty

BibTex


@article{BUT165708,
  author="Pavel {Neužil}",
  title="The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond",
  annote="Infectious diseases, such as the most recent case of coronavirus disease 2019, have brought the prospect of point-of-care (POC) diagnostic tests into the spotlight. A rapid, accurate, low-cost, and easy-to-use test in the field could stop epidemics before they develop into full-blown pandemics. Unfortunately, despite all the advances, it still does not exist. Here, we critically review the limited number of prototypes demonstrated to date that is based on a polymerase chain reaction (PCR) and has come close to fulfill this vision. We summarize the requirements for the POC PCR tests and then go on to discuss the PCR product-detection methods, the integration of their functional components, the potential applications, and other practical issues related to the implementation of lab-on-a-chip technologies. We conclude our review with a discussion of the latest findings on nucleic acid-based diagnosis. (C) 2020 Elsevier B.V. All rights reserved.",
  address="ELSEVIER SCI LTD",
  chapter="165708",
  doi="10.1016/j.trac.2020.115984",
  howpublished="online",
  institution="ELSEVIER SCI LTD",
  number="115984",
  volume="130",
  year="2020",
  month="september",
  pages="115984--115984",
  publisher="ELSEVIER SCI LTD",
  type="journal article in Web of Science"
}