Detail publikace

Detection of Highly Variable Genome Fragments in Unmapped Reads of Escherichia coli Genomes

NYKRÝNOVÁ, M. BARTOŇ, V. BEZDÍČEK, M. LENGEROVÁ, M. ŠKUTKOVÁ, H.

Originální název

Detection of Highly Variable Genome Fragments in Unmapped Reads of Escherichia coli Genomes

Anglický název

Detection of Highly Variable Genome Fragments in Unmapped Reads of Escherichia coli Genomes

Jazyk

en

Originální abstrakt

Whole-genome sequencing becomes a powerful tool in the study of closely related bacterial population as a single nucleotides changes can be detected. However, the postprocessing of obtained data still remains problematic. Reference-based assembly of genomes only allows identification of shared parts of genomes. Here, we show a pipeline for de novo assembly of unmapped reads and locating variable regions in it. Identified regions can be used as new markers for bacterial genotyping.

Anglický abstrakt

Whole-genome sequencing becomes a powerful tool in the study of closely related bacterial population as a single nucleotides changes can be detected. However, the postprocessing of obtained data still remains problematic. Reference-based assembly of genomes only allows identification of shared parts of genomes. Here, we show a pipeline for de novo assembly of unmapped reads and locating variable regions in it. Identified regions can be used as new markers for bacterial genotyping.

Dokumenty

BibTex


@inproceedings{BUT163867,
  author="Markéta {Nykrýnová} and Vojtěch {Bartoň} and Matěj {Bezdíček} and Martina {Lengerová} and Helena {Škutková}",
  title="Detection of Highly Variable Genome Fragments in Unmapped Reads of Escherichia coli Genomes",
  annote="Whole-genome sequencing becomes a powerful tool in the study of closely related bacterial population as a single nucleotides changes can be detected. However, the postprocessing of obtained data still remains problematic. Reference-based assembly of genomes only allows identification of shared parts of genomes. Here, we show a pipeline for de novo assembly of unmapped reads and locating variable regions in it. Identified regions can be used as new markers for bacterial genotyping.",
  booktitle="Bioinformatics and Biomedical Engineering",
  chapter="163867",
  doi="10.1007/978-3-030-45385-5_51",
  howpublished="print",
  year="2020",
  month="may",
  pages="569--578",
  type="conference paper"
}