Detail publikace

Multi-electrode array with a planar surface for cell patterning by microprinting

SLAVÍK, J. SKOPALÍK, J. PROVAZNÍK, I. HUBÁLEK, J.

Originální název

Multi-electrode array with a planar surface for cell patterning by microprinting

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.

Klíčová slova

multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent

Autoři

SLAVÍK, J.; SKOPALÍK, J.; PROVAZNÍK, I.; HUBÁLEK, J.

Vydáno

5. 12. 2019

Nakladatel

MDPI

ISSN

1424-8220

Periodikum

SENSORS

Ročník

19

Číslo

24

Stát

Švýcarská konfederace

Strany od

1

Strany do

11

Strany počet

11

URL

https://www.mdpi.com/1424-8220/19/24/5379

Plný text v Digitální knihovně

http://hdl.handle.net/11012/184124

BibTex

@article{BUT161298,
  author="Jan {Slavík} and Josef {Skopalík} and Valentine {Provazník} and Jaromír {Hubálek}",
  title="Multi-electrode array with a planar surface for cell patterning by microprinting",
  journal="SENSORS",
  year="2019",
  volume="19",
  number="24",
  pages="1--11",
  doi="10.3390/s19245379",
  issn="1424-8220",
  url="https://www.mdpi.com/1424-8220/19/24/5379"
}