Detail publikace

Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

AMIRMANSOOR, A. SÝS, M. SEDLÁČKOVÁ, E. FARAG, A. ADAM, V. PŘIBYL, J. RICHTERA, L.

Originální název

Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.

Klíčová slova

glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition

Autoři

AMIRMANSOOR, A.; SÝS, M.; SEDLÁČKOVÁ, E.; FARAG, A.; ADAM, V.; PŘIBYL, J.; RICHTERA, L.

Vydáno

3. 7. 2019

Nakladatel

MDPI

ISSN

1424-8220

Periodikum

SENSORS

Ročník

19

Číslo

13

Stát

Švýcarská konfederace

Strany od

1

Strany do

15

Strany počet

15

URL

https://www.mdpi.com/1424-8220/19/13/2939

Plný text v Digitální knihovně

http://hdl.handle.net/11012/180521

BibTex

@article{BUT158050,
  author="Amirmansoor {Ashrafi} and Milan {Sýs} and Eliška {Dostálová} and Amir Shaaban {Farag} and Vojtěch {Adam} and Jan {Přibyl} and Lukáš {Richtera}",
  title="Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals",
  journal="SENSORS",
  year="2019",
  volume="19",
  number="13",
  pages="1--15",
  doi="10.3390/s19132939",
  issn="1424-8220",
  url="https://www.mdpi.com/1424-8220/19/13/2939"
}