Detail publikace

Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma

Originální název

Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma

Anglický název

Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma

Jazyk

en

Originální abstrakt

Our approach aims at applying the electron spin resonance (ESR) spectroscopy to a complex biological system and establishing a semi-invasive method of detection of human microRNA (miRNA) in renal cell carcinoma. Nowadays, detection of miRNAs´ presence in cancer cells is done by performing quantitative polymerase chain reaction (qPCR) which monitors gene amplification of a target sequence by attaching fluorescent chemical compounds. Herein, we are focused on gaining an insight into the physical and chemical environment by using a spin probe, namely a nitroxide spin label (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) directly attached to anti-hsa-miR-21-5p oligonucleotides.

Anglický abstrakt

Our approach aims at applying the electron spin resonance (ESR) spectroscopy to a complex biological system and establishing a semi-invasive method of detection of human microRNA (miRNA) in renal cell carcinoma. Nowadays, detection of miRNAs´ presence in cancer cells is done by performing quantitative polymerase chain reaction (qPCR) which monitors gene amplification of a target sequence by attaching fluorescent chemical compounds. Herein, we are focused on gaining an insight into the physical and chemical environment by using a spin probe, namely a nitroxide spin label (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) directly attached to anti-hsa-miR-21-5p oligonucleotides.

BibTex


@misc{BUT157611,
  author="Jakub {Hrubý} and Petr {Neugebauer}",
  title="Electron Spin Resonance Detection of Human MicroRNA 21-5p in Renal Cell Carcinoma",
  annote="Our approach aims at applying the electron spin resonance (ESR) spectroscopy to a complex biological system and establishing a semi-invasive method of detection of human microRNA (miRNA) in renal cell carcinoma. Nowadays, detection of miRNAs´ presence in cancer cells is done by performing quantitative polymerase chain reaction (qPCR) which monitors gene amplification of a target sequence by attaching fluorescent chemical compounds. Herein, we are focused on gaining an insight into the physical and chemical environment by using a spin probe, namely a nitroxide spin label (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) directly attached to anti-hsa-miR-21-5p oligonucleotides.",
  address="Masarykova Univerzita",
  booktitle="Joint Retreat 2019",
  chapter="157611",
  howpublished="print",
  institution="Masarykova Univerzita",
  year="2019",
  month="june",
  pages="53--53",
  publisher="Masarykova Univerzita",
  type="abstract"
}