Detail publikace

Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles

MODLITBOVÁ, P. NOVOTNÝ, K. POŘÍZKA, P. FARKA, Z. ZLÁMALOVÁ GARGOŠOVÁ, H. ROMIH, T. KAISER, J.

Originální název

Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles

Anglický název

Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles

Jazyk

en

Originální abstrakt

In this work, we focused on several analytical instrumental methods utilization and the benefits of their outcomes in phytotoxicity assessment. Two model plants - Allium cepa L. and Lemna minor L. were used to test five types of cadmium Quantum Dots (QDs). In the first study, L. minor was used to test cadmium telluride QDs capped with two coating ligands: glutathione (GSH) or 3-mercaptopropionic acid (MPA). Macroscopic toxicity endpoints number of fronds and fresh weight of L. minor were determined after 168-hour exposure and growth rate inhibition and final biomass inhibition were evaluated. The next objective was to determine total content of Cd in plants using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). Laser-Induced Breakdown Spectroscopy (LIBS) with 200 µm lateral resolution was employed to obtain two-dimensional maps of Cd spatial distribution in L. minor fronds. Finally, L. minor fronds were subjected to transmission electron microscopy (TEM) to examine QDs ability to penetrate cell walls. In the A. cepa phytotoxicity study, cadmium telluride QDs with or without shells were tested, i.e. 2-mercaptopropionic acid capped CdTe, CdTe/ZnS, and CdTe/CdS/ZnS QDs. CdCl2 served as positive control. After 24-hour and 72-hour exposure, the total length of the root system was measured. Then the total Cd content in different plant tissues (roots, bulbs, and shoots) was determined by ICP-OES. A. cepa roots were examined by fluorescence microscopy to determine adsorption of Cd-based QDs on the root surface and its relation to the QD type. Free Cd2+ content in exposure media before and after the test was measured by differential pulse voltametry (DPV) to assess differences in chemical stability among the QD types.

Anglický abstrakt

In this work, we focused on several analytical instrumental methods utilization and the benefits of their outcomes in phytotoxicity assessment. Two model plants - Allium cepa L. and Lemna minor L. were used to test five types of cadmium Quantum Dots (QDs). In the first study, L. minor was used to test cadmium telluride QDs capped with two coating ligands: glutathione (GSH) or 3-mercaptopropionic acid (MPA). Macroscopic toxicity endpoints number of fronds and fresh weight of L. minor were determined after 168-hour exposure and growth rate inhibition and final biomass inhibition were evaluated. The next objective was to determine total content of Cd in plants using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). Laser-Induced Breakdown Spectroscopy (LIBS) with 200 µm lateral resolution was employed to obtain two-dimensional maps of Cd spatial distribution in L. minor fronds. Finally, L. minor fronds were subjected to transmission electron microscopy (TEM) to examine QDs ability to penetrate cell walls. In the A. cepa phytotoxicity study, cadmium telluride QDs with or without shells were tested, i.e. 2-mercaptopropionic acid capped CdTe, CdTe/ZnS, and CdTe/CdS/ZnS QDs. CdCl2 served as positive control. After 24-hour and 72-hour exposure, the total length of the root system was measured. Then the total Cd content in different plant tissues (roots, bulbs, and shoots) was determined by ICP-OES. A. cepa roots were examined by fluorescence microscopy to determine adsorption of Cd-based QDs on the root surface and its relation to the QD type. Free Cd2+ content in exposure media before and after the test was measured by differential pulse voltametry (DPV) to assess differences in chemical stability among the QD types.

Dokumenty

BibTex


@proceedings{BUT151872,
  author="Pavlína {Modlitbová} and Karel {Novotný} and Pavel {Pořízka} and Helena {Zlámalová Gargošová} and Jozef {Kaiser}",
  title="Utilization of analytical instrumental methods in phytotoxicity testing of nanoparticles",
  annote="In this work, we focused on several analytical instrumental methods utilization and the benefits of their outcomes in phytotoxicity assessment. Two model plants - Allium cepa L. and Lemna minor L. were used to test five types of cadmium Quantum Dots (QDs).
In the first study, L. minor was used to test cadmium telluride QDs capped with two coating ligands: glutathione (GSH) or 3-mercaptopropionic acid (MPA). Macroscopic toxicity endpoints number of fronds and fresh weight of L. minor were determined after 168-hour exposure and growth rate inhibition and final biomass inhibition were evaluated. The next objective was to determine total content of Cd in plants using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). Laser-Induced Breakdown Spectroscopy (LIBS) with 200 µm lateral resolution was employed to obtain two-dimensional maps of Cd spatial distribution in L. minor fronds. Finally, L. minor fronds were subjected to transmission electron microscopy (TEM) to examine QDs ability to penetrate cell walls.
In the A. cepa phytotoxicity study, cadmium telluride QDs with or without shells were tested, i.e. 2-mercaptopropionic acid capped CdTe, CdTe/ZnS, and CdTe/CdS/ZnS QDs. CdCl2 served as positive control. After 24-hour and 72-hour exposure, the total length of the root system was measured. Then the total Cd content in different plant tissues (roots, bulbs, and shoots) was determined by ICP-OES. A. cepa roots were examined by fluorescence microscopy to determine adsorption of Cd-based QDs on the root surface and its relation to the QD type. Free Cd2+ content in exposure media before and after the test was measured by differential pulse voltametry (DPV) to assess differences in chemical stability among the QD types.",
  booktitle="Book of abstracts of the ISEAC‐40 Internaonal Conference on Environmental & Food  Monitoring",
  chapter="151872",
  howpublished="online",
  year="2018",
  month="june",
  pages="380--380",
  type="conference proceedings"
}