Detail publikace

Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake

PEŠKOVÁ, M. HEGER, Z. DOSTÁLOVÁ, S. FOJTŮ, M. ČÁSTKOVÁ, K. ILKOVICS, L. VYKOUKAL, V. PEKAŘÍK, V.

Originální název

Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Ceria nanoparticles can catalyze many redox reactions that resemble reactions catalyzed by naturally occurring enzymes, such as peroxidase, catalase, haloperoxidase or phosphatase. This makes nanoceria an attractive material for biomedical and sensing applications. Presence of detergents dramatically affects the catalytic activity of nanoceria. The catalytic activity is also strongly influenced by biogenic compounds present in the sample. Furthermore, biomedical applications are hindered by the difficulties to detect nanoceria uptake by cells. Therefore, we have developed a new fast and sensitive fluorescent haloperoxidase assay based on fluorescein bromination in presence of CTAB to detect uptake of CeO2 nanoparticles by cells in vitro. Moreover, catalytic activities of standard nanoceria (CeO2) were compared to nanoceria hydrates (CeO2.xH2O) that are easier for preparation and might be stabilized by biologically relevant molecules allowing for a specific targeting to cells or tissues. We have developed a new approach to produce CeO2.xH2O in presence of TEMED and analyzed whether the presence of H2O2 during the synthesis influences the catalytic activity of produced particles.

Klíčová slova

Detergents · fluorescent detection · haloperoxidase, nanoceria hydrate · TEMED

Autoři

PEŠKOVÁ, M.; HEGER, Z.; DOSTÁLOVÁ, S.; FOJTŮ, M.; ČÁSTKOVÁ, K.; ILKOVICS, L.; VYKOUKAL, V.; PEKAŘÍK, V.

Vydáno

28. 9. 2018

ISSN

2365-6549

Periodikum

ChemistrySelect

Ročník

3

Číslo

36

Stát

Spolková republika Německo

Strany od

10139

Strany do

10146

Strany počet

8

BibTex

@article{BUT151469,
  author="Marie {Pešková} and Zbyněk {Heger} and Simona {Dostálová} and Michaela {Fojtů} and Klára {Částková} and Ladislav {Ilkovics} and Vít {Vykoukal} and Vladimír {Pekařík}",
  title="Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake",
  journal="ChemistrySelect",
  year="2018",
  volume="3",
  number="36",
  pages="10139--10146",
  doi="10.1002/slct.201802496",
  issn="2365-6549"
}