Detail publikace

Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors

Originální název

Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors

Anglický název

Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors

Jazyk

en

Originální abstrakt

Polyhydroxyalkanoates (PHAs) are hydrophobic polyesters produced and accumulated by numerous microorganisms in a form of intracellular granules filled with the polymer and covered by a layer of proteins. The polymer content of bacteria can reach up to 90 % of the weight of cellular dry matter. In our recent work, we have confirmed experimentally that PHAs granules do not serve only as a source of carbon or energy but also as a cell component with a complex protective performance. PHAs accumulating bacteria cells are synthesizing and degrading the polymers simultaneously. We have confirmed that the monomer of PHAs, which is found in the polymer-accumulating bacterial in a substantial amount, serves as a chemical chaperone and a cryo- protective solute. Furthermore, we have revealed that the protective function of the polymer against various stressors such as non-optimal pH, high and low temperature, osmotic up- and down-shock, UV exposure etc. is conditioned by the native amorphous state of PHAs. This liquid-like state of PHAs, found only in-vivo, is involved in the adaptation of cells supporting them to survive sudden changes of the environment.

Anglický abstrakt

Polyhydroxyalkanoates (PHAs) are hydrophobic polyesters produced and accumulated by numerous microorganisms in a form of intracellular granules filled with the polymer and covered by a layer of proteins. The polymer content of bacteria can reach up to 90 % of the weight of cellular dry matter. In our recent work, we have confirmed experimentally that PHAs granules do not serve only as a source of carbon or energy but also as a cell component with a complex protective performance. PHAs accumulating bacteria cells are synthesizing and degrading the polymers simultaneously. We have confirmed that the monomer of PHAs, which is found in the polymer-accumulating bacterial in a substantial amount, serves as a chemical chaperone and a cryo- protective solute. Furthermore, we have revealed that the protective function of the polymer against various stressors such as non-optimal pH, high and low temperature, osmotic up- and down-shock, UV exposure etc. is conditioned by the native amorphous state of PHAs. This liquid-like state of PHAs, found only in-vivo, is involved in the adaptation of cells supporting them to survive sudden changes of the environment.

Dokumenty

BibTex


@misc{BUT151441,
  author="Eva {Slaninová} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Polyhadroxyalkanoates –microbial polyesters with a complex protective effect against diverse stress factors",
  annote="Polyhydroxyalkanoates (PHAs) are hydrophobic polyesters produced and accumulated by numerous microorganisms in a form of intracellular granules filled with the polymer and covered by a layer of proteins.  The polymer content of bacteria can reach up to 90 % of the weight of cellular dry matter. In our recent work, we have confirmed experimentally that PHAs granules do not serve only as a source of carbon or energy but also as a cell component with a complex protective performance. PHAs accumulating bacteria cells are synthesizing and degrading the polymers simultaneously. We have confirmed that the monomer of PHAs, which is found in the polymer-accumulating bacterial in a substantial amount, serves as a chemical chaperone and a cryo- protective solute. Furthermore, we have revealed that the protective function of the polymer against various stressors such as non-optimal pH, high and low temperature, osmotic up- and down-shock, UV exposure etc. is conditioned by the native amorphous state of PHAs. This liquid-like state of PHAs, found only in-vivo, is involved in the adaptation of cells supporting them to survive sudden changes of the environment.",
  address="Tiskárna Didot, spol. s. r. o.",
  booktitle="XIX. setkání biochemiků a molekulárních biologů",
  chapter="151441",
  howpublished="print",
  institution="Tiskárna Didot, spol. s. r. o.",
  year="2018",
  month="november",
  pages="44--44",
  publisher="Tiskárna Didot, spol. s. r. o.",
  type="abstract"
}