Detail publikace

Spectral analysis of human norepinephrine transporter homing peptides

HADDAD, Y. MILOSAVLJEVIĆ, V. NEJDL, L. RICHTERA, L. HEGER, Z. ADAM, V.

Originální název

Spectral analysis of human norepinephrine transporter homing peptides

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

UV spectrometry is very simple and cheap method for quantitative and qualitative analysis of compounds. Furthermore, it provides intricate information about the bonded -electron transitions and also non-bonded n-electron transitions. The aim of this work was to identify electron transition bands in two homing peptides of the human norepinephrine transporter (hNET); namely: GASNGINAYL (978 Da) and SLWERLAYGI (1206 Da). Electron transition bands directly indicate structural conformations, particularly the ones associated with double bonds, i.e. conjugated -bonds of aromatic and peptide bonds. The results show unusual spikes in absorbance in the far UV at low temperature for GASNGINAYL and even more at other temperatures for SLWERLAYGI peptide. The latter supports the hypothesis of a stacking between tyrosine and tryptophan resulting in helix structure. Infrared spectrometry also showed abundant helix structure in SLWERLAYGI but less abundant in GASNGINAYL peptide. Based on -stacking, an UV spectrometry method can be developed to monitor the helicity of some peptides, such as SLWERLAYGI.

Klíčová slova

UV spectrometry; peptide; tyrosine; tryptophan; targeted therapy

Autoři

HADDAD, Y.; MILOSAVLJEVIĆ, V.; NEJDL, L.; RICHTERA, L.; HEGER, Z.; ADAM, V.

Vydáno

31. 12. 2017

ISBN

978-80-7509-529-9

Kniha

MendelNet 2017

Strany od

867

Strany do

872

Strany počet

6

BibTex

@inproceedings{BUT148725,
  author="Yazan Abdulmajeed Eyadh {Haddad} and Vedran {Milosavljević} and Lukáš {Nejdl} and Lukáš {Richtera} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Spectral analysis of human norepinephrine transporter homing peptides",
  booktitle="MendelNet 2017",
  year="2017",
  pages="867--872",
  isbn="978-80-7509-529-9"
}