Detail publikace

Isolation of reduced and oxidized glutathione using maghemite particles

CERNEI, N. KOPEL, P. MILOSAVLJEVIĆ, V. HORKÝ, P. ADAM, V.

Originální název

Isolation of reduced and oxidized glutathione using maghemite particles

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Here we report the optimal conditions for the isolation of reduced glutathione (GSH) and oxidized glutathione (GSSG) from various types of matrix, such as blood, plasma or cells and their characterization using ionexchange liquid chromatography with VIS detector. In our study, we aimed to synthesize paramagnetic microparticles able to isolate and immobilize GSH, GSSG from blood or plasma and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified amberlite and Dowex by nanomaghemite (γ-Fe2O3), as the functional carriers providing perfect paramagnetic properties. Both the paramagnetic microparticles were able to bind GSH and GSSG. Our paramagnetic microparticles have potential to improve GSH and GSSG isolation and in future can serve for application as a platform of biosensors or delivery system.

Klíčová slova

Ion exchange chromatography; maghemite microparticles; glutathione; reactive oxygen species

Autoři

CERNEI, N.; KOPEL, P.; MILOSAVLJEVIĆ, V.; HORKÝ, P.; ADAM, V.

Vydáno

31. 3. 2018

ISBN

978-80-87294-81-9

Kniha

NANOCON 2017

Edice

1st Edition, 2018

Strany od

165

Strany do

169

Strany počet

5

BibTex

@inproceedings{BUT148688,
  author="Natalia Vladimirovna {Cernei} and Pavel {Kopel} and Vedran {Milosavljević} and Pavel {Horký} and Vojtěch {Adam}",
  title="Isolation of reduced and oxidized glutathione using maghemite particles",
  booktitle="NANOCON 2017",
  year="2018",
  series="1st Edition, 2018",
  pages="165--169",
  isbn="978-80-87294-81-9"
}