Detail publikace

ELECTROCHEMICAL DETECTION OF QUANTUM DOTS USED AS A TOOL FOR LABELLING INFLUENZA DERIVED OLIGONUCLEOTIDE

KREJČOVÁ, L. HYNEK, D. KOPEL, P. ADAM, V. HUBÁLEK, J. KIZEK, R.

Originální název

ELECTROCHEMICAL DETECTION OF QUANTUM DOTS USED AS A TOOL FOR LABELLING INFLUENZA DERIVED OLIGONUCLEOTIDE

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

The aim of this study was designed hybridization assay consisting of probe modified magnetic particles (MPs) and specific oligonucleotide labeled by quantum dots (QDs), and their application in the detection of avian influenza virus (H5N1) respectively influenza derived oligonucleotide (ODN). There were two basic steps used in the assay: (i) isolation of specific oligonucleotide (target) by anti-sense bounded MPs and (ii) target detection by QDs marker. It was optimized the efficiency of hybridization temperatures between anti senses and target influenza oligonucleotide. QDs labeled target was detected by electrochemical analysis. Our results show that MPs based automatic isolation together with electrochemical detection is available tool for influenza detection.

Klíčová slova

voltammetry; quantum dots; influenza; oligonucleotide; nanoparticles; automatic isolation hybridization assay; electrochemistry

Autoři

KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.

Vydáno

22. 11. 2012

ISBN

978-80-7375-836-3

Kniha

MENDELNET 2012

Strany od

1233

Strany do

1243

Strany počet

11

BibTex

@inproceedings{BUT145625,
  author="Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam} and Jaromír {Hubálek} and René {Kizek}",
  title="ELECTROCHEMICAL DETECTION OF QUANTUM DOTS USED AS A TOOL FOR LABELLING INFLUENZA DERIVED OLIGONUCLEOTIDE",
  booktitle="MENDELNET 2012",
  year="2012",
  pages="1233--1243",
  isbn="978-80-7375-836-3"
}