Detail publikace

Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers

GUMULEC, J. MASAŘÍK, M. KŘÍŽKOVÁ, S. HLAVNA, M. BABULA, P. HRABEC, R. ROVNÝ, A. MASAŘÍKOVÁ, M. SOCHOR, J. ADAM, V. ECKSCHLAGER, T. KIZEK, R.

Originální název

Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Current diagnostic techniques are inefficient in distinguishing latent and low-risk forms of prostate cancer from high-risk forms. The present study is focused on determination of putative tumor markers of aggressive high-grade forms of prostate cancer. Potential markers were determined in blood sera of 133 patients (82 cases and 51 controls) and in cell lines (Gleason score 9-derived 22Rv1 and normal tissue derived PNT1A) on mRNA and protein levels. Alpha-methylacyl-CoA racemase (AMACR), metallothionein classes 1A and 2A (MT1A and MT2A) were determined and compared to prostate specific antigen (PSA) levels. On mRNA level, significantly increased expression of MT2A (2.4-fold), PSA (2.6-fold) and AMACR (8.4-fold) and insignificantly (1.9-fold) elevated MT1A in 22Rv1 compared to non-tumor PNT1A were determined. On protein level, significant enhancement of free PSA and total PSA in tumor cell line was evident. AMACR protein was 1.5-fold elevated in tumor line (below the level of significance). Contrary to mRNA, significantly (p = 0.01) reduced level of MT protein in tumor lines was determined. In the case of serum level, significantly enhanced MT level (4.5-fold) in patients' sera was found. No significant changes were observed in the case of AMACR. These findings indicate possible alternative role of MT to PSA prostate cancer marker. In addition, level of AMACR is distinctly higher in the Gleason score 9 in serum of patients and MT shows a descending trend in relation to Gleason score.

Klíčová slova

cancer; tumor marker; immunodetection; electrochemistry; polymerase chain reaction; mRNA

Autoři

GUMULEC, J.; MASAŘÍK, M.; KŘÍŽKOVÁ, S.; HLAVNA, M.; BABULA, P.; HRABEC, R.; ROVNÝ, A.; MASAŘÍKOVÁ, M.; SOCHOR, J.; ADAM, V.; ECKSCHLAGER, T.; KIZEK, R.

Vydáno

1. 12. 2012

ISSN

0028-2685

Periodikum

Neoplasma

Ročník

59

Číslo

2

Stát

Slovenská republika

Strany od

191

Strany do

200

Strany počet

10

BibTex

@article{BUT145125,
  author="Jaromír {Gumulec} and Michal {Masařík} and Soňa {Křížková} and Marian {Hlavna} and Petr {Babula} and Roman {Hrabec} and Arne {Rovný} and Monika {Masaříková} and Jiří {Sochor} and Vojtěch {Adam} and Tomáš {Eckschlager} and René {Kizek}",
  title="Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers",
  journal="Neoplasma",
  year="2012",
  volume="59",
  number="2",
  pages="191--200",
  doi="10.4149/neo\{_}2012\{_}025",
  issn="0028-2685"
}