Detail publikace

Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa

FOJT, P. ZLÁMALOVÁ GARGOŠOVÁ, H.

Originální název

Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa

Anglický název

Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa

Jazyk

en

Originální abstrakt

The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluating possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we choosed two different life stages of this snail: eggs and juvenile individuals. The evaluated substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs, embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the endpoints were: mass and shell diameter. Second, the exposition of eggs was performed in small petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. This test lasted for 14 days and the endpoint was the percentage of successfully hatched snails. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes. The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of environmental toxicants.

Anglický abstrakt

The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluating possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we choosed two different life stages of this snail: eggs and juvenile individuals. The evaluated substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs, embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the endpoints were: mass and shell diameter. Second, the exposition of eggs was performed in small petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. This test lasted for 14 days and the endpoint was the percentage of successfully hatched snails. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes. The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of environmental toxicants.

Dokumenty

BibTex


@misc{BUT143930,
  author="Pavel {Fojt} and Helena {Zlámalová Gargošová}",
  title="Ecotoxicity Assessment of Cadmium Using Different Life Stages of the Terrestrial Gastropod Helix aspersa aspersa",
  annote="The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluating possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we choosed two different life stages of this snail: eggs and juvenile individuals. The evaluated substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs, embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the endpoints were: mass and shell diameter. Second, the exposition of eggs was performed in small petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. This test lasted for 14 days and the endpoint was the percentage of successfully hatched snails. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes.
The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of environmental toxicants.

",
  booktitle="Book of Abstracts of the18th European Meeting on Environmental Chemistry - EMEC18:
Chemistry Towards an Infinite Environment",
  chapter="143930",
  edition="1",
  howpublished="print",
  year="2017",
  month="november",
  pages="158--158",
  type="presentation"
}